Endofitske bakterije pospešujejo rast in toleranco na sol riža v razmerah slanosti

Trung Quang DO, Anh The LUU, Chuong Tien NGO

Povzetek


Razvoj biostimulantov iz bakterij, ki pospešuejo rast na sol tolerantnih rastlin (PGP) je razvijajoča se strategija za vzdržnostno kmetijstvo v času naraščajočega zasoljevanja tal. Namen raziskave je bil izolirati endofitske bakterije (EB), ki so sposobne pospeševati kalitev semen riža in rast sejank pri različnih koncentracijah NaCl. Izoliranih je bilo devet na sol tolerantnih sevov EB in dva seva, ST.6 in ST.8, ki sta pospeševala kalitev semen riža za 99,3 in 99,7 %. Ta dva seva sta bila izolirana in določena kot vrsti Pantoea dispersa in Burkholderia cenocepacia. Sev ST.6 je pokazal večjo vrednost v aktivnosti fosfataze (617 mg P ml-1), v produkciji indole-3-ocetne kisline (19,7 µg IAA ml-1), v aktivnosti deaminaze 1-aminociklopropan-1-karboksilne kisline (ACC) (13,5 µmol mg−1 protein h−1) in v tvorbi sideroforov (76,3 %). Še posebej so sejanke riža, inokulirane s sevom ST.6, pokazale značilno izboljšanje v dolžini korenin (58,95 %), dolžini poganjkov (16,6 %), suhi biomasi (7,0 %), v vsebnosti klorofila (46,2 in 57,1 % za klorofil a in b), karotenoidov (22,2 %) in prolina (19,0 %). V sejankah riža, inokuliranih s sevom ST.6 ali ST.8, je bil opazen tudi upad aktivnosti antioksidacijskih encimov v razmerah solnega stresa. Nadalje so razmere solnega stresa pospešile kolonizacijo korenin z obema sevoma preučevanih endofitskih bakterij. Še več poskusov bo potrebnih, da bi razvili seva endofitskih bakterij ST.6 in ST.8 kot učinkovita bioinokulanta.


Ključne besede


endofiti; solni stres; bakterije, ki pospešujejo rast rastlin; antioksidacijski encimi; kalitev semen; riž (Oryza sativa)

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2023.119.1.2899

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Avtorske pravice (c) 2023 Quang Trung DO, The Anh LUU, Tien Chuong NGO

 

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