VPLIV SALICILNE KISLINE NA NEKATERE FIZIOLOŠKE ODZIVE VRSTE PELINA (Artemisia aucheri Boiss.) NA SUŠNI STRES V IN VITRO RAZMERAH

Jalil ABBASPOUR, Aliakbar EHSANPOUR

Povzetek


Salicilna kislina je pomemben rastlinski hormon, ki je vključen v uravnavanje rasti, razvoja in odziva na stres. Cilj raziskave je bil ovrednotiti nekatere fiziološke in biokemične odzive vrste pelina Artemisia aucheri Boiss. v sušnem stresu po zunanjem dodajanju salicilne kisline. Poskus je potekal in vitro, v MS gojišču (Murashige and Skoog., 1962) z dodatkom polietilen glikola (PEG/6000, 0, 2 in 4 % (w/v)) za simulacijo sušnega stresa in dvema različnima koncentracijama salicilne kisline (SA) (0, 0.01 in 0.1 mM). Po štirih tednih je salicilna kislina zmanjšala negativne učinke polietilen glikola na suho in svežo maso kot tudi na vsebnosti klorofila in karotenoidov. V sušnem stresu je uporaba salicilne kisline zmanjšala vsebnost založnih polisaharidov in povečala vsebnost topnih ogljikovih hidratov. Čeprav polietilen glikol ni imel značilnega učinka na vsebnostn flavonoidov je značilno povečal vsebnost antocianinov, celokupnih fenolov, celokupno antioksidacijsko sposobnost, aktivnost PAL (fenilalanin amonium-liaza) in TAL (tirozin amonium-liaza), obravnavanje s salicilno kislino je te parametre značilno izboljšalo. Glede na te rezultate je bilo zaključeno, da salicilna kislina povečuje strpnost na sušo pri vrsti Artemisia aucheri s povečanjem biosinteze fenolnih snovi, z izboljšanjem aktivnosti TAL in PAL kot tudi s povečanjem vsebnosti topnih ogljikovih hidratov.

Ključne besede


Artemisia aucheri Boiss., salicilna kislina, sušni stres; polietilen glikol, rast, fenilalanin amonium-liaza, tirozin amoniium-liaza

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2016.107.2.03

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