Sonifikacija semen sezama z ultrazvokom poveča njihovo kalitev v razmerah hladnega stresa

Fariborz SHEKARI, Seyyed-Hamid MUSTAFAVI, Amin ABBASI

Povzetek


V laboratorijskem poskusu smo določali učinke ultrazvoka (US) na kalitev semen sezama. Vsi poskusi so bili izvedenei v ultrazvočni vodni kopeli s frekvenco ultrazvoka 20 kHz , pri čemer smo spreminjali dva dejavnika in sicer trajanje obdelave z ultrazvokom (10, 20 in 30 min) in temperaturo kalitve (15, 20 in 25 ºC). Vzporedno so potekali poskusi, v katerih so bila semena samo namočena v vodi brez ultrazvočne sonifikacije, da bi odpravili učinke vode pri ultrazvočno obdelanih semenih. Obdelava semen z ultrazvokom je v njih povečala privzem vode. Pri srednjih obravnavanjih z ultrazvokom se je izboljšala kalitev in rast kalic pri suboptimalnih temperaturah, kar se je odrazilo kot večji odstotek kalitve in njen hitrejši potek. US obdelava za 20 min je rahlo povečala aktivnost superoksid dizmutase, vendar v primerjavi s kontrolo in obdelavi z US 10 min ni bilo značilnih razlik. Aktivnost katalaze se je pri obdelavah z 10 in 20 min močno povečala. Obdelava z ultrazvokom za 10 in 20 min je zmanjšala vsebnost malondialdehida in H2O2, obdelava za 30 min pa je vsebnost obeh parametrov povečala. Na splošno lahko na osnovi te raziskave povzamemo, da je ultrasonična predobdelava semen sezama koristna tehnika za njegovo zgodnjo setev, kar vodi v večje pridelke.

Ključne besede


sezam; sonifikacija; seme; encimska aktivnost; kalitev; rast kalic; ultrazvok; privzem vode

Celotno besedilo:

PDF (English)

Literatura


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DOI: http://dx.doi.org/10.14720/aas.2015.105.2.03

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