Celulosomi in celulazni konzorcij Ruminococcus flavefaciens 007C

Maša VODOVNIK

Povzetek


Ruminococcus flavefaciens sodi med najpomembnejše celulolitične bakterijske vrste v vampu prežvekovalcev in debelem črevesu monogastričnih rastlinojedih sesalcev. Njena izjemna učinkovitost pri anaerobni razgradnji (hemi)celuloznih substratov je povezana s sintezo zunajceličnih multiencimskih kompleksov celulosomov. V pričujočem delu smo preučili genomski potencial za sintezo celulosomov in celulaz seva 007C, izražanje slednjih pa analizirali z encimogrami. V osnutku genoma tega seva smo identificirali v celoti ohranjeno gručo genov, ki kodirajo skafoldine, na osnovi njihove modularne zgradbe pa smo predvideli zgradbo celulosomov tega seva. Nabor genov za celulaze, ki smo jih identificirali v genomu seva 007C, obsega po en zapis za encima s katalitskima domenama GH48 in GH44 ter širok spekter domnevnih endoglukanaz iz družin GH9 in GH5. Pri večini teh encimov smo identificirali dokerinske domene, kar kaže na to, da so vezani v celulosome. Module za vezavo na celulozo smo identificirali le v kombinaciji s katalitskimi domenami GH9. Ugotovili smo tudi, da se večje endoglukanaze izražajo konstitutivno, manjše encime pa smo zaznali le v supernatantu kultur, ki so daljši čas rasle na mikrokristalinični celulozi.

Ključne besede


mikrobiologija; molekularna genetika; Ruminococcus flavefaciens; celulosomi; glikozid hidrolaze; anaerobna razgradnja celuloze

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2014.104.2.5

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