Anliza vzorca beljakovin v odporni in občutljivi sorti pšenice v razmerah slanostnega stresa

Marouf KHALILI, Mohammad Reza NAGHAVI, Said YOUSEFZADEH

Povzetek


Za analizo proteomskega vzorca v dveh sortah pšenice so bili vzorčeni mladi listi v fazi bilčenja dva tedna po izpostavitvi slanostnemu stresu. Izvleček beljakovin iz listnih tkiv je bil narejen z dvodimenzionalno elektroforezo z uporabo IPG trakov in SDS-PAGE, za rastline iz kontrole in tiste v slanostnem stresu. Celokupno je bilo evidentiranih 198 beljakovinskih točk za odporno sorto (‘Moghan3’) in 203 beljakovinskih točk za občutljivo sorto (‘Pishtaz’). Med temi sta bili ugotovljeni beljakovinski točki št. 21 in 22 z značilnimi vrednostmi IF za ‘Moghan3’ in ‘Pishtaz’. Za določitev beljakovin v točkah je bila uporabljena dvofazna masna spektrometrija (MS/MS). Določene beljakovine so obsegale encime, ki so vključeni pri odstranjevanju oksidantov, encime Kalvinovega cikla, beljakovine, ki so udeležene v svetlobnih reakcijah fotosinteze in v protonskem transportu ter beljakovine vročinskega udara. Beljakovine so bile določene na osnovi funkcionalnih skupin in njihove frekvence. V splošnem je v stresnih razmerah odporna sorta ‘Moghan3’ ohranjala stabilnost zgradbe in poteka presnove ogljika bolje kot občutljiva sorta. Dodatno sta se za obrambo proti oksidacijskem stresu v razmerah slanosti inducirala dva proteina, 2-cis peroksiredoksin BAS1 in Cu-Zn SOD protein, ki sta odporno vrsto ščitila bolje kot občutljivo. Odpornost sorte ‘Moghan3’ in občutljivost sorte ‘Pishtaz’ na slanostni stres temelji na edinstvenem vorcu beljakovin, ki se tvorijo v razmerah slanosti za odpravljanje oksidantov in vzdrževanje aktivnosti svetlobnih rekacij fotosinteze.

Ključne besede


proteomska analiza; odpornost na sol; stresni proteini; dvodimenzionalna elektroforeza; pšenica

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2018.111.3.03

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