Nekateri pomembni vidiki v mikropropagaciji moringe (Moringa oleifera Lam.)

Abdullah EL-NAGISH, Ahmed HASSANEIN, Jehan SALEM, Fayza FAHEED

Povzetek


Vir in vrsta izsečkov kot tudi vrsta citokininov sta pomembna dejavnika za uspešno mikropropagacijo moringe. Izsečki, pridobljeni iz in vitro vzgojenih rastlin so bili boljši kot tisti pridobljeni iz sejank vzgojenih v tleh. Dodatno so bili izsečki nodijev boljši kot iz ostalih delov poganjka glede na število nastalih poganjkov na izseček, pogostost tvorbe poganjkov in število nodijev na poganjek. Tvorba kalusa na bazi nodialnega segmenta je bila boljša v gojišču z BAP kot v gojišču s KIN, še posebej zaradi vpliva večjih koncentracij je bil pri mikropropagaciji moringe z vidika vitrifikacije BAP boljši kot KIN. Gojišče z majhno vsebnostjo hranil  (polovični MS) z dodatkom 0,5 mg l-1 IAA je bilo odločilno za tvorbo korenin. Primerne razmere za mikropropagacijo moringe na polnomočnih gojiščih MS ali SH so manj stresne in ne vzpodbudijo tvorbo SOD in POX. Po drugi strani je stres zaradi povečanja spojin v dvojnem gojišču MS ali v polovičnih gojiščih MS, B5 ali WPM z majhno količino hranil vzpodbudil največjo ekspresijo SOD in POX , kar se je pokazalo z največjim številom in močnejšo obarvanostjo eletroforeznih trakov, obratno je bilo ugotovljeno v primeru CAT.

Ključne besede


tip gojišča; razmnoževanje poganjkov; citokinini; izražanje genov; izoencimi; mikropropagacija; antioksidacijski encimi; vrsta izsečkov

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2019.113.1.02

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