UČINEK SLANOSTI NA VRSTO Brassica rapa var. toria (BRSRT) OB PRISOTNOSTI SELENA: POSKUS OVREDNOTENJA ZAŠČITNE VLOGE SELENA

Akanksha SAO, Priya SARAF, Divya BAGCHI

Povzetek


V raziskavi je ovrednotena vloga selena kot antioksidanta pri rastlini v slanostnem stresu. Vpliv natrijevega selenata (Na2SeO4) na rast, oksidacijski stres in antioksidacijsko zaščito vrste Brassica rapa var. toria (BRSRT) je bil preučevan v hidroponskem poskusu. 40 µmol in 100 µmol Na2SeO4 je bilo 12 dni hidroponsko dodajano koreninam rastline, ki so rastle v 50 mmol in 100 mmol raztopini natrijevega klorida (NaCl). Rast rastlin, produkcija biomase in vsebnost fotosinteznih pigmentov so bili zavrti pri rastlinah pod 100 mmol solnim stresom, aktivirani pa so bili indikatorji oksidativnega stresa kot sta vodikov peroksid in peroksidacija lipidov. Dodajanje 40 µmol Na2SeO4 pri 50 mmol in 100 mmol NaCl je izboljšalo rast, vsebnost fotosinteznih barvil in delovalo kot antioksidant z inhibicijo peroksidacije lipidov in povečanjem aktivnosti superoksid dismutase, askorbat peroksidaze, katalaze, glutation peroksidaze in glutation reduktaze. Povečano aktivnost teh encimov je pokazala tudi njihova gelska anliza. Selen je pri koncentraciji 100 µmol v razmerah solnega stresa zavrl rast in tvorbo antioksidativnih encimov in vzpobudil oksidacijski stres s povečano aktivnostjo glutation peroksidaze. V danih stresnih obravnavanjih je bilo dodajanje 40 µmol Na2SeO4 najbolj učinkovito pri obeh koncentracijah NaCl. Rezultati kažejo, da predstavlja optimalno dodajanje selena dober obet za njegovo uporabo v razmerah relativno velikega NaCl stresa pri sadikah obravnavane rastline.

Ključne besede


Brassica rapa; antioksidanti; slanostni stres; natrijev selenat

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2017.109.3.09

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