Izražanje transferinskih genov pri postrvih (Salmo sp.)

Anja ČIBEJ, Simona SUŠNIK BAJEC

Povzetek


Družina salmonidov združuje sladkovodne in anadromne vrste rib. Za salmonide je značilno, da imajo podvojene mnoge lokuse v genomski DNA, nekatere kot posledica tetraploidizacije genoma, druge kot posledica neodvisne podvojitve posameznih regij DNA. Pri rodu Salmo so dokazali podvojenost transferinskega gena pri atlantskem lososu, potočni in soški postrvi. Namen dela je bila karakterizacija promotorske regije obeh genov (TF1, TF2) pri vseh treh vrstah in določitev razmerja v izražanju TF1 in TF2 pri atlantskem lososu. S qPCR smo dokazali, da se pri atlantskem lososu TF2 izraža šestkrat šibkeje od TF1; predhodno je bilo dokazano, da je razlika v izražanju obeh genov pri potočni in soški postrvi še večja. Določili smo nukleotidno zaporedje različno dolgim promotorskim regijam obeh različic gena pri treh vrstah. V promotorski regiji smo našli mikrosatelit, ki se razlikuje v dolžini tako med vrstami kot med genoma, in štiri SNP, ki so opredeljevale TF1 in TF2. Pri atlantskem lososu smo določili zaporedje daljšega odseka promotorske regije. V promotorju gena za TF1 atlantskega lososa se nahaja minisatelit, ki obsega 37 bp dolg motiv s preko 20 ponovitvami, medtem ko pri TF2 minisatelita ni. Pri analizi potencialnih vezavnih mest smo pri vseh promotorskih regijah našli pomembne regije za izražanje transferinskega gena.


Ključne besede


ribe; atlantski losos; potočna postrv; soška postrv; genetika; transferinski geni; izražanje genov; promotorske regije

Celotno besedilo:

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Literatura


Allendorf, F. W., & Thorgaard, G. H. (1984). Tetraploidy and the evolution of Salmonid fishes. V: B. J. Turner (ur.), Evolutionary Genetics of fishes (str. 1–53). Virginia: Plenum Press.

Andersen, Ø., DeRosa, M. C., Pirolli, D., Tooming-Klunderud, A., Petersen, P.E., & Andre, C. (2011). Polymorphism, selection and tandem duplication of transferrin genes in Atlantic cod (Gadus morhua) – Conserved synteny between fish monolobal and tetrapod bilobal transferrin loci. BMC Genetics, 12(51), 14. https://doi.org/10.1186/1471-2156-12-51

Antunes, A., Gharbi, K., Alexandrino, P., & Guyomard, R. (2006). Characterization of transferrin-linked microsatellites in brown trout (Salmo trutta) and Atlantic salmon (Salmo salar). Molecular ecology notes, 6(2), 547–549. https://doi.org/10.1111/j.1471-8286.2005.01253.x

Baker, H. M., Anderson, B. F., & Baker, E. N. (2003). Dealing with iron: Common structural principles in proteins that transport iron and heme. Proceedings of the National Academy of Sciences, 100, 3579–3583. https://doi.org/10.1073/pnas.0637295100

Barnes, D., & Sato, G. (1980). Methods for growth of cultured cells in serum-free medium. Analytical Biochemistry, 102, 255–270. https://doi.org/10.1016/0003-2697(80)90151-7

Brunel, F., Ochoa, A., Schaeffer, E., Boissier, F., Guillou, Y., Cereghini, S., ... Zakin, M. M. (1988). Interactions of DNA-binding proteins with 5’ region of the human transferrin gene. The journal of biological chemistry, 263(21), 10180–10186.

Bullen, J. J., Rogers, H. J., Spalding, P. B., & Ward, C. G. (2006). Natural resistance, iron and infection: a challenge for clinical medicine. Journal of medical microbiology, 55, 251–258. https://doi.org/10.1099/jmm.0.46386-0

Ellis, A. E. (2001). Innate host defense mechanisms of fish against viruses and bacteria. Developmental and Comparative Immunology, 25, 827–839. https://doi.org/10.1016/S0145-305X(01)00038-6

Faisst, S., & Meyer, S. (1992). Compilation of vertebrate-encoded transcription factors. Nucleic Acids Research, 20, 3–26. https://doi.org/10.1093/nar/20.1.3

Ford, M. J. (2001). Molecular evolution of transferrin: Evidence for positive selection in salmonids. Molecular biology and evolution, 18(4), 639–647. https://doi.org/10.1093/oxfordjournals.molbev.a003844

Ford, M. J., Thornton, P. J., & Park, L. K. (1999). Natural selection promotes divergence of transferrin among salmonid species. Molecular Ecology, 8(6), 1055–1061. https://doi.org/10.1046/j.1365-294x.1999.00651.x

Kvingedal, A. M. (1994). Characterization of the 5’ region of the Atlantic salmon (Salmo salar) transferrin-encoding gene. Gene, 150, 335–339. https://doi.org/10.1016/0378-1119(94)90448-0

Kvingedal, A. M., & RØrvik, K. A. (1993). Cloning and characterization of Atlantic salmon (Salmo salar) serum transferrin cDNA. Molecular marine biology and biotechnology, 2(4), 233–238.

Lamb, P., & McKnlght, S. L. (1991). Diversity and specificity in transcriptional regulation: The benefits of heterotypic dimerization. Trends in Biochemical Sciences, 16, 417–422. https://doi.org/10.1016/0968-0004(91)90167-T

Lambert, L. A., Perri, H., & Meehan, T. J. (2005). Evolution of duplications of the transferrin family of proteins. Comparative Biochemistry and Physiology, B, 140, 11–25. https://doi.org/10.1016/j.cbpc.2004.09.012

Liang, G. M., & Jiang, X. P. (2010). Positive selection drives lactoferrin evolution in mammals. Genetica, 138, 757–762. https://doi.org/10.1007/s10709-010-9456-x

Lynch, M., & Conery, J. S. (2000). The evolutionary fate and consequences of duplicated genes. Science, 290(5494), 1151–5. https://doi.org/10.1126/science.290.5494.1151

Mainou-Fowler, T., & Brock, J. H. (1985). Effect of iron deficiency on the response of mouse lymphocytes to concavalin A: The importance of transferrin-bound iron. Immunology, 54, 325–332.

MatInspector. (2013). MatInspector: Search for transcription factor binding sites. Genomatix. Pridobljeno s https://www.genomatix.de/online_help/help_matinspector/matinspector_help.html

Miller, S. A., Dykes, D. D., & Polesky, H. F. (1988). A simple salting out procedure from human nucleated cells. Nucleic Acids Research, 16, 1215. https://doi.org/10.1093/nar/16.3.1215

Ohno, S. (1970). Evolution by gene duplication. New York: Springer-Verlag. https://doi.org/10.1007/978-3-642-86659-3

Phillips, R. B., & Oakley, T. H. (1997). Phylogenetic relationships among the Salmoninae based on nuclear and mitochondrial DNA sequences. V T. D. Kocher & C. A. Stepien (ur.), Molecular systematics of fishes (str. 145–162), New York: Academic Press.

Real Time PCR Handbook. (2003). RRC Core Genomics Facility, University of Illinois at Chicago. Pridobljeno s

https://www.gene-quantification.de/real-time-pcr-handbook-life-technologies-update-flr.pdf

Rozman, T. (2008). Opis transferinskega lokusa in njegova uporaba pri filogenetskih analizah rodu Salmo (doktorska disertacija). Ljubljana: Medicinska fakulteta.

Rozman, T., Dovč, P., Marić, S., Kokalj-Vokač, N., Erjavec-Škerget, A., Rab, P., & Snoj, A. (2008). Evidence for two transferrin loci in the Salmo trutta genome. Animal genetics, 39, 577–585. https://doi.org/10.1111/j.1365-2052.2008.01768.x

Sawaya, S. M., Lennon, D., Buschiazzo, E., & Gemmell, N. (2012). Promoter microsatellites as modulators of human gene expression. V A. J. Hannan (ur.), Tandem repeat polymorphism: Genetic plasticity, neutral diversity and disease. Austin, Texas: Landes Biosciences, Springer Science+Business Media.

Schaeffer, E., Guillou, F., Part, D., & Zakin, M. M. (1993). A different combination of transcription factors modulates the expression of the human transferrin promoter in liver and Sertoli cells. The Journal of Biological Chemistry, 268, 23399–23408.

Sun, Y., Zhu, Z., Wang, R., Sun, Y., & Xu, T. (2012). Miiuy croaker transferrin gene and evidence for positive selection events reveal different evolutionary patterns. Plos one, 7(9), 9. https://doi.org/10.1371/journal.pone.0043936

Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599. https://doi.org/10.1093/molbev/msm092

Wang, G., & Vasquez, K. M. (2007). Z-DNA, an active element in the genome. Frontiers in Bioscience, 12, 4424–4438. https://doi.org/10.2741/2399

Woo, P. T. K., & Ardelli, B. F. (2014). Immunity against selected piscine flagellates. Developmental and comparative immunology, 43, 268–279. https://doi.org/10.1016/j.dci.2013.07.006

Zhang, J. (2003). Evolution by gene duplication: an update. Trends in ecology and evolution, 18, 292–298. https://doi.org/10.1016/S0169-5347(03)00033-8




DOI: http://dx.doi.org/10.14720/aas.2018.112.1.4

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