Ločevanje hibridnih starševskih linij riža z RAPD in ISSR markerji

Ghaffar KIANI, Camellia KATALANI

Povzetek


Genetska oddaljenost med starševskimi linijami riža, ki so bile uporabljene v programu vzgoje hibridnega riža, je bila ocenjena na osnovi informacij iz podatkov, pridobljenih z molekularnimi markerji. Šestnajst staršev (5 CMS, 5 vzdrževalnih linij (vzdrževalci) in 6 linij, ki povrnejo fertilnost (obnovitelji) je bilo analiziranih s 15 RAPD in 20 ISSR markerji. Med 15 RAPD markerji jih je bilo 9 polimorfnih. Teh 9 markerjev je pomnožilo 79 fragmentov, med katerimi jih je bilo 28 polimorfnih (35 %). Z uporabo 10 od 20 ISSR markerjev so pomnožili 86 fragmentov, od katerih je bilo 35 polimorfnih (41 %). Indeks markerjev (RAPD = 1,68; ISSR = 1,88) in odstotek polimorfnih fragmentov sta pokazala, da so bili ISSR markerji relativno uspešnejši pri ugotavljanju polimorfnosti. Klasterska analiza staršev, ki je temeljila na Jaccardovi genetski podobnosti in na UPGMA metodi je pokazala tri skupine. Liniji obnoviteljev IR68061 in IR5931, z izvorom na Filipinih, sta tvorili jasno ločeno skupino. Rezulti nakazujejo, da bi križance med linijama CMS Neda in Nemat z linijama obnoviteljev IR68061 in IR5931 lahko uporabili kot najboljšo skupino križancev pri uporabi tega načina žlatnenja.

Ključne besede


riž; obnovitelej (restorer); vzdrževalec (maintainer); klasterska analiza; molekularni marker

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2018.111.2.12

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