CTAB protokol za ekstraskcijo RNK iz melise (Melissa officinalis L.)

Fatemeh RAHMANI, Leila AMRAEE

Povzetek


Kakovost in ohranjenost ribonukleinske kisline (RNK) sta bistveni za mnoge raziskave v molekularni biologiji rastlin. Visoko kakovostni izvlečki RNK iz rastlin z veliko vsebnostjo spojin kot so polisaharidi, polifenoli in drugi sekundarni metaboliti so problematični. Ekstrakcija RNK iz tkiv melise je lahko težavna zaradi prisotnosti polifenolov in polisaharidov ali pa je lahko narejena le z dragimi protokoli. Raziskava predstavlja izboljšanje protokola na osnovi CTAB, ki omogoča hitro in enostavno izolacijo kvalitetne RNK iz melise. Pridobljena RNK je primerna za cDNK sintezo in RT-PCR poskuse.


Ključne besede


Melissa officinalis; CTAB; RNK; RT-PCR

Celotno besedilo:

PDF (English)

Literatura


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DOI: http://dx.doi.org/10.14720/aas.2020.115.1.692

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