Vpliv rastlinskih rastnih regulatorjev in salicilne kisline na tvorbo nekaterih sekundarnih metabolitov v kalusu in suspenziji celične kulture vrste šetraja Satureja sahendica Bornm.

Sarieh TARIGHOLIZADEH, Rouhollah MOTAFAKKERAZAD, Morteza KOSARI-NASAB, Ali MOVAFEGHI, Sakineh MOHAMMADI, Mohsen SABZI, Amir-Hossein TALEBPOUR

Povzetek


Preučevan je bil vpliv kombinacije rastlinskih rastnih regulatorjev (PGRs) na kulturo kalusa vrste šetraja Satureja sahendica Bornm. V nodijskih izsečkih je bil preučevan odziv tvorbe sekundarnih metabolitov na različne koncentracije PGRs glede na prisotnost in odsotnost polivinilpirolidona (PVP). Izsečki so bili gojeni v MS gojišču v prisotnosti auksinov (2,4-diklorofenoksiocetna kislina in naftilocetna kislina) in citokininov (tidiazuron in kinetin), ki so bili uporabljeni v enakih koncentracijah 0,5; 1, in 2 mg l-1. Obravnavanje 2 mg l-1 2,4-D + 2 mg l-1 Kin (MD3) je vodilo k največji tvorbi celokupnih fenolov (4,303 ± 0,449 mg GAE g-1) in flavonoidov (2,903 ± 7,016 mg QE g-1). Dodatno je bil ovrednoten učinek salicilne kisline (SA) na tvorbo sekundarnih metabolitov v kulturi suspenzije celic šetraja. Kultura suspenzije celic je bila vzpostavljena z gojenjem rahlega kalusa, pridobljenga iz nodijskih izsečkov v tekočem mediju, ki je vseboval različne koncentracije SA (0, 100, 150, 200 µM). Pojavilo se je obratno sorazmerje med svežo maso in vsebnostjo sekundarnih metabolitov. Med različnimi koncentracijami salicilne kisline ni bilo opaziti značilnih razlik na tvorbo celokupnih fenolov in flavonoidov. Salicilna kislina vzpodbuja tvorbo sekundarnih metabolitov in zmajšuje svežo maso celične kulture.

Ključne besede


Satureja sahendica; indukcija kalusa; kultura suspenzije celic; sekundarni metaboliti; rastni regulatorji; salicilna kislina

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2021.117.4.773

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