Izražanje na sol preobčutljivega gena (SOS1) bi pri pšenici v razmerah slanosti lahko uravnavali s sevom bakterije Azospirillum brasilense Sp7

Hamid Reza GHASSEMI, Akbar MOSTAJERAN, Abolghasem ESMAEILI

Povzetek


Slanostni stres zmanjšuje rast rastlin preko odpovedi fizioloških procesov v glavnem zaradi Na+ iona. Preobčutljiva (SOS) solna signalna pot predstavlja najvažnejši del Na+/K+ homeostaznega sistema v rastlinah v razmerah slanosti. Še več, poroča se, da je povezava pšenice z bakterijo iz rodu Azospirillum rezultirala v povečani odpornosti na slanost. Za ovrednotenje vloge bakterije iz rodu Azospirillum pri uravnavanju SOS signalne poti so bile gojene z bakterijo Azospirillum brasilense Sp7 inokulirane in neinokulirane sejanke pšenice za pet dni. Izenačene sejanke so bile prenešene v hidroponski medij z ali brez 200 mM NaCl. V presledkih 6, 24 in 48 ur po prenosu sejank v slan medij je bila merjena relativna ekspresija gena TaSOS1 v koreninah, listni nožnici in listni ploskvi in Na+/K+ razmerje. Hkrati je bila 72 ur po prenosu v slan medij izmerjena vsebnost Ca, Fe in prolina, suha masa pogankov in vsebnost topnih sladkorjev v istih delih sejank. Rezultati so pokazali, da je slanost povečala ekspresijo gena TaSOS1, vsebnost Na+, prolina in povečala količnik Na+/K+, a zmanjšala vsebnost Ca in Fe v koreninah in poganjkih sejank. Čeprav lahko bakterija A. brasilense Sp7 izboljša toleranco pšenice na slanost z zmanjšanjem privzema Na in povečano ekspresijo gena TaSOS1 pa nima nobenega učinka na razporeditev natrija v rastlini. Slanost torej lahko poveča ekspresijo gena TaSOS1 v koreninah in listih in inokulacija z bakterijo A. brasilense Sp7 lahko zmanjša škodljive učinke slanosti preko dodatnega vpliva na povečano ekspresijo TaSOS1.

Ključne besede


Azospirillum; pšenica; slanost; TaSOS1; Na+/ K+ razmerje

Celotno besedilo:

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Literatura


Akbarimoghaddam, H., Galavi, M., Ghanbari, A., & Panjehkeh, N. (2011). Salinity effects on seed germination and seedling growth of bread wheat cultivars, Trakia journal of Sciences, 9(1), 43-50.

Amini, F., Askary, M., Haghir, M., & Ghassemi, H. R. (2017). Changes in antioxidant system and oxidative stress under water stress in four cucumber cultivars. Indian Journal of Plant Physiology, 22(1), 114-119. doi:10.1007/s40502-017-0285-0

Amooaghaie, R., Mostajeran, A., & Emtiazi, G. (2002). The effect of compatible and incompatible Azospirillum brasilense strains on proton efflux of intact wheat roots, Plant and soil, 243(2),155-160.

Ardakani, M.R., Mazaheri, D., Mafakheri, S., & Moghaddam, A. (2011). Absorption efficiency of N, P, K through triple inoculation of wheat (Triticum Aestivum L.) by Azospirillum brasilense, Streptomyces sp., Glomus intraradices and manure application, Physiology and molecular biology of plants : an international journal of functional plant biology, 17(2), 181-192.

Askary, M., Mostajeran, A., & Emtiazi, G. (2008). Colonization and nitrogenase activity of Triticum aestivum (cv. Baccross and Mahdavi) to the dual inoculation with Azospirillum brasilense and Rhizobium meliloti plus 2,4-D. Pakistan Journal of Biological Sciences, 11(12), 1541-1550.

Askary, M., Mostajeran, A., Amooaghaei, R., & Mostajeran, M. (2009). Influence of the co-inoculation Azospirillum brasilense and Rhizobium meliloti plus 2, 4-D on grain yield and N, P, K content of Triticum aestivum (cv. Baccros and Mahdavi), American-Eurasian Journal Agriculture Environment Science, 5, 296-307.

Askary, M., Talebi, S. M., Amini, F., & Bangan, A. D. B. (2017). Effects of iron nanoparticles on Mentha piperita L. under salinity stress. Biologija, 63(1), 65-75.

Baniaghil, N., Arzanesh, M., Ghorbanli, M., & Shahbazi, M. (2013). The effect of plant growth promoting rhizobacteria on growth parameters, antioxidant enzymes and microelements of canola under salt stress, J Appl Environment Biology Science, 3, 17-27.

Bates, L., Waldren, R., & Teare, I. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205-207. doi:10.1007/BF00018060

Camilios-Neto, D., Bonato, P., Wassem, R., Tadra-Sfeir, M.Z., Brusamarello-Santos, L.C.C., Valdameri, G., Donatti, L., Faoro, H., Weiss, V.A., Chubatsu, L.S., Pedrosa, F.O., & Souza, E.M. (2014). Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes, BMC Genomics, 15(1), 378.

Creus, C.M., Sueldo, R.J., & Barassi, C.A. (1997). Shoot growth and water status in Azospirillum-inoculated wheat seedlings grown under osmotic and salt stresses, Plant physiology and biochemistry-paris, 35, 939-944.

Davenport, R., James, R. A., Zakrisson-Plogander, A., Tester, M., & Munns, R. (2005). Control of sodium transport in durum wheat. Plant Physiology, 137(3), 807-818. doi:10.1104/pp.104.057307

Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances, Analytical Chemistry, 28(3), 350-356.

El-Dengawy, E., Hussein, A.A., & Alamri, S.A. (2011). Improving growth and salinity tolerance of carob seedlings (Ceratonia siliqua L.) by Azospirillum inoculation, American-Eurasian Journal of Agricultural & Environmental Sciences, 11(3), 371-384.

El-Hendawy, S.E., Hu, Y., Yakout, G.M., Awad, A.M., Hafiz, S.E., & Schmidhalter, U. (2005). Evaluating salt tolerance of wheat genotypes using multiple parameters, European journal of agronomy, 22 (3), 243-253.

Feki, K., Brini, F., Ben Amar, S., Saibi, W., & Masmoudi, K. (2014). Comparative functional analysis of two wheat Na+/H+ antiporter SOS1 promoters in Arabidopsis thaliana under various stress conditions, Journal of Applied Genetics, 56(1), 15-26.

Fraile ‐Escanciano, A., Kamisugi, Y., Cuming, A. C., Rodríguez‐Navarro, A., & Benito, B. (2010). The SOS1 transporter of Physcomitrella patens mediates sodium efflux in planta. New Phytologist, 188(3), 750-761. doi:10.1111/j.1469-8137.2010.03405.x

Hamdia, M.A.E.-S., Shaddad, M., & Doaa, M.M. (2004). Mechanisms of salt tolerance and interactive effects of Azospirillum brasilense inoculation on maize cultivars grown under salt stress conditions, Plant Growth Regulation, 44(2), 165-174.

Heuer, B. (2010). Role of proline in plant response to drought and salinity, Handbook of plant and crop stress. CRC Press, Boca Raton, 213-238.

Hoagland, D.R., & Arnon, D.I. (1950). The water-culture method for growing plants without soil, Circular. California Agricultural Experiment Station, 347 (2nd edit).

Kang, S.-M., Khan, A.L., Waqas, M., You, Y.-H., Kim, J.-H., Kim, J.-G., Hamayun, M., & Lee, I.-J. (2014). Plant growth-promoting rhizobacteria reduce adverse effects of salinity and osmotic stress by regulating phytohormones and antioxidants in Cucumis sativus, Journal of Plant Interactions, 9(1), 673-682.

Lekshmy, S., Sairam, R., & Kushwaha, S. (2013). Effect of long-term salinity stress on growth and nutrient uptake in contrasting wheat genotypes. Indian Journal of Plant Physiology, 18(4), 344-353. doi:10.1007/s40502-014-0059-x

Maghsoudi, K., & Arvin, M.J. (2010). Salicylic acid and osmotic stress effects on seed germination and seedling growth of wheat (Triticum aestivum L.) cultivars, World Applied Sciences Journal, 2(1), 7-11.

Mostajeran, A., & Gholaminejad, A. Effect of salinity on sodium & potassium uptake and proline, carbohydrates contents of turmeric plant parts, Journal of Current Chemical & Pharmaceutical Sciences, 4(1) (1), 10-21.

Nadeem, S.M., Zahir, Z.A., Naveed, M., Arshad, M., & Shahzad, S. (2006). Variation in growth and ion uptake of maize due to inoculation with plant growth promoting rhizobacteria under salt stress, Soil Environment, 25 (2), 78-84.

Neumann, P.M. (1995). The role of cell wall adjustments in plant resistance to water deficits, Crop Science, 35 (5), 1258-1266.

Norastehnia, A., Niazazari, M., Sarmad, J., & Rassa, M. (2014). Effects of chloride salinity on non-enzymatic antioxidant activity, proline and malondialdehyde content in three flue-cured cultivars of Tobacco, Journal of Plant Development, 21.

Öğüt, M., Akdağ, C., Düzdemir, O., & Sakin, M.A. (2005). Single and double inoculation with Azospirillum/Trichoderma: the effects on dry bean and wheat, Biology and Fertility of Soils, 41(4), 262-272.

Omar, M.N.A., Osman, M.E.H., Kasim, W.A., & Abd El-Daim, I.A. (2009). Improvement of salt tolerance mechanisms of barley cultivated under salt stress using Azospirillum brasilense, chapter title, in: Ashraf, M., Ozturk, M., Athar, H.R. (Eds.), Salinity and Water Stress: Improving Crop Efficiency. Springer Netherlands, Dordrecht, pp. 133-147.

doi:10.1007/978-1-4020-9065-3_15

Pakdaman, N., Mostajeran, A., & Hojati, Z. (2014). Phosphate concentration alters the effective bacterial quorum in the symbiosis of Medicago truncatula-Sinorhizobium meliloti, Symbiosis, 62(3), 151-155.

Pessarakli, M., & Huber, J. (1991). Biomass production and protein synthesis by alfalfa under salt stress, Journal of Plant Nutrition, 14(3), 283-293.

Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT–PCR, Nucleic Acids Research, 29(9), e45-e45.

Ramezani, A., Niazi, A., Abolimoghadam, A.A., Babgohari, M.Z., Deihimi, T., Ebrahimi, M., Akhtardanesh, H., & Ebrahimie, E. (2013). Quantitative expression analysis of TaSOS1 and TaSOS4 genes in cultivated and wild wheat plants under salt stress, Molecular Biotechnology, 53(2), 189-197.

Sathee, L., Sairam, R. K., Chinnusamy, V., & Jha, S. K. (2015). Differential transcript abundance of salt overly sensitive (SOS) pathway genes is a determinant of salinity stress tolerance of wheat. Acta physiologiae plantarum, 37(8), 169-179. doi:10.1007/s11738-015-1910-z

Shi, H., Quintero, F.J., Pardo, J.M., & Zhu, J.-K. (2002). The putative plasma membrane Na+/H+ antiporter SOS1 controls long-distance Na+ transport in plants, The Plant Cell, 14(2), 465-477.

Shi, H., & Zhu, J.-K. (2002). Regulation of expression of the vacuolar Na+/H+ antiporter gene AtNHX1 by salt stress and abscisic acid, Plant molecular biology, 50(3), 543-550.

Tavakoli, M., Poustini, K., & Alizadeh, H. (2016). Proline accumulation and related genes in wheat leaves under salinity stress. Journal of Agricultural Science and Technology, 18(3), 707-716.

Tiwari, J.K., Munshi, A.D., Kumar, R., Pandey, R.N., Arora, A., Bhat, J.S., & Sureja, A.K. (2010). Effect of salt stress on cucumber: Na+/K+ ratio, osmolyte concentration, phenols and chlorophyll content, Acta physiologiae plantarum, 32(1), 103-114.

Turan, N.G. (2008). The effects of natural zeolite on salinity level of poultry litter compost, Bioresource Technology, 99(7), 2097-2101.

Upadhyay, S., Singh, J., & Singh, D. (2011). Exopolysaccharide-producing plant growth-promoting rhizobacteria under salinity condition. Pedosphere, 21(2), 214-222. doi:10.1016/S1002-0160(11)60120-3

Upadhyay, S.K., Singh, J.S., Saxena, A.K., & Singh, D.P. (2012). Impact of PGPR inoculation on growth and antioxidant status of wheat under saline conditions, Plant Biology, 14(4), 605-611.

Vargas, L., de Carvalho, T.L.G., Ferreira, P.C.G., Baldani, V.L.D., Baldani, J.I., & Hemerly, A.S. (2012). Early responses of rice (Oryza sativa L.) seedlings to inoculation with beneficial diazotrophic bacteria are dependent on plant and bacterial genotypes, Plant and Soil, 356(1), 127-137.

Wakeel, A. (2013). Potassium–sodium interactions in soil and plant under saline-sodic conditions, Journal of Plant Nutrition and Soil Science, 176(3), 344-354.

Xu, H., Jiang, X., Zhan, K., Cheng, X., Chen, X., Pardo, J.M., & Cui, D. (2008). Functional characterization of a wheat plasma membrane Na+/H+ antiporter in yeast, Archives of Biochemistry and Biophysics, 473(1), 8-15.

Xue, Z.-Y., Zhi, D.-Y., Xue, G.-P., Zhang, H., Zhao, Y.-X., & Xia, G.-M. (2004). Enhanced salt tolerance of transgenic wheat (Tritivum aestivum L.) expressing a vacuolar Na+/H+ antiporter gene with improved grain yields in saline soils in the field and a reduced level of leaf Na+, Plant Science, 167(4), 849-859.

Yadav, N.S., Shukla, P.S., Jha, A., Agarwal, P.K., & Jha, B. (2012). The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na+loading in xylem and confers salt tolerance in transgenic tobacco, BMC Plant Biology, 12, 188-188.

Zarea, M., Hajinia, S., Karimi, N., Goltapeh, E.M., Rejali, F., & Varma, A. (2012). Effect of Piriformospora indica and Azospirillum strains from saline or non-saline soil on mitigation of the effects of NaCl, Soil Biology and Biochemistry, 45, 139-146




DOI: http://dx.doi.org/10.14720/aas.2018.111.2.17

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