Vpliv temperaturnega stresa na profil in fitotoksičnost sekundarnih metabolitov v listnem izvlečku zrnatega ščira (Amaranthus cruentus L.)

Maria Elizabeth CAWOOD, Ingrid ALLEMANN, James ALLEMANN

Povzetek


Rastline zrnatega ščira so bile za namene te raziskave gojene v nadzorovanih optimalnih temperaturnih razmerah tri mesece (28/21 °C) in nato izpostavljene hladu (14/7 °C) in vročini (33/40 °C). Preučevan je bil vpliv temperaturnih režimov na profil metabolitov v listih ščira z metodami kot so TLC, HPLC in GC-MS spektroskopija. Fitotoksični potencial metanolno-vodnih (MW) in diklormetanskih (DCM) izvlečkov nadzemnih delov ščira je bil analizirana preko in vitro analize kalitve vrtne solate in paprike. Optimalni izvlečki so imeli največjo raznolikost sekundarnih metabolitov in največjo vsebnost celokupnih fenolov in flavonoidov. S TLC in HPLC analizo je bila potrjena značilno manjša vsebnost fenolov v vročinsko obdelanih vzorcih. Z GC-MS analizo je bil ugotovljen širok nabor metabolitov v diklormetanskih izvlečkih (DCM). Fitotoksičnost MW in DCM izvlečkov se je izrazila v značilno zmanjšani kalitvi in rasti solate in paprike, kar kaže na prisotnost več kot ene alelokemične spojine. To lahko posledično vpliva na alelopatsko aktivnost zrnatega ščira med spremembami temperature v okolju.

Ključne besede


Amaranthus cruentus; temperatura; stres; fitotoksičnost; metaboliti; fenolne spojine

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2018.111.3.09

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