Učinki dodajanja prolina na fiziološke lastnosti krušne pšenice (Triticum aestivum L.) in navadne leče( Lens culinaris Medik.) v razmerah sušnega stresa

Selma BEKKA, Ouzna ABROUS-BELBACHIR, Réda DJEBBAR

Povzetek


Proline je kot indikator stresa pogosto uporabljen za testiranje odpornosti rastlin na sušo. Z dodajanjem prolina se je mogoče izogniti škodljivim učinkom suše na rast rastlin. Predmet te raziskave je bil preučiti vpliv dodajanja prolina v rastni medij na fiziologijo dveh rastlinskih vrst, krušne pšenice kot enokaličnice in navadne leče kot dvokaličnice, gojenih v razmerah sušnega stresa. Po predhodnih poskusih sta bili določeni optimalni koncentraciji prolina, 6 mM za krušno pšenico in 2 mM za navadno lečo. Obe vrsti sta bili obravnavani s prolinom v normalnih in v sušnih razmerah. Rezultati so pokazali, da je pomanjkanje vode prizadelo obe vrsti, kar je vodilo v zmanšano rast, manjšo vsebnost klorofila in zmanjšano relativno vsebnost vode. Podobno je obravnavanje s 15 % PEG-6000, kar je enakovredno osmotskemu potencialu -0.31MPa, povzročilo veliko kopičenje prolina v obeh vrstah. Opažen je bil tudi znaten upad v aktivnosti katalaze (Cat) in askorbat perkosidaze (APX), kar je bilo verjetno posledica oksidacijskega stresa, povzročenega s sušo. Uporaba prolina je pri obeh vrstah zmanjšala škodljive učinke sušnega stresa z akumulacijo endogenega prolina, kar je tudi zmanšalo aktivnosti Cat, APX in GPX.

Ključne besede


Triticum aestivum L.; Lens culinaris Medik.; eksogeni prolin; odpornost na sušo

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2018.111.2.20

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