Vpliv različnih gojitvenih gojišč na rast transformiranih korenin zdravilne špajke (Valeriana officinalis L.)

Ali PAKDIN PARIZI, Mohammad FARSI, Ghorban-Ali NEMATZADEH, Amin MIRSHAMSI

Povzetek


Transformirana kultura korenin zdravilne špajke je bila vzpostavljena z bakterijsko okužbo Agrobacterium rhizogenes, sev ATCC 15834. Preučevana so bila različna gojišča MS, B5 (1,0X in 0,5X koncentracija), N6 in modificiran MS brez fitohormonov. Dodatno so bila v MS gojišču preučevana različna razmerja med NH4+ in NO3-. Učinki teh tretmajev na rast transformiranih korenin so bili ovrednoteni po 21 dneh. B5 in ½ B5 sta bili najboljši osnovni gojišči za rast transformiranih korenin. MS gojišče dopolnjeno z dušikovimi spojinami v razmerju 20:20 (mM) NH4+: NO3- je vplivalo na največjo rast in biomaso korenin. Raziskava je pokazala, da imata sestava rastnega gojišča in različna razmerja dušikovih spojin značilen vpliv na rast transformiranih korenin zdravilne špajke.

Ključne besede


Valeriana officinalis; zdravilne rastline; zdravilna špajka; transformirane korenine; sestava rastnega gojišča

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2014.103.2.14

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