Primerjava diagnostičnih metod spremljanja pojava raka (Agrobacterium vitis Ophel & Kerr) žlahtne vinske trte (Vitis vinifera L.) v Sloveniji

Janja LAMOVŠEK, Igor ZIDARIČ, Irena MAVRIČ PLEŠKO, Gregor UREK, Stanislav TRDAN

Povzetek


Bakterija Agrobacterium vitis je povzročitelj raka žlahtne vinske trte (Vitis vinifera L). Ta bolezen je uničila mnogo slovenskih vinogradov pred več kot desetimi leti. V sklopu spremljanja pojava bolezni smo leta 2006 izolirali 80 izolatov, ki smo jih s PCR metodama pehA in multipleks določili kot vrsti A. vitis in A. tumefaciens. Sposobnost sevov, da izzovejo nastanek tumorjev, smo ocenjevali na gostiteljskih rastlinah. Z molekularnimi metodami pa smo določali prisotnost plazmida Ti, povzročitelja nastanka tumorjev. Z metodo PCR smo ugotovili šest lažno negativnih patogenih sevov in nobenega lažno pozitivnega. Velika genetska raznolikost patogenih sevov Agrobacterium spp. vpliva na zanesljivost določanja z molekularnimi metodami, zato se v primeru dvomljivih rezultatov priporoča dodatna izvedba bioloških testov na rastlinah.

Ključne besede


vinska trta; vitis vinifera; Agrobacterium vitis; gostiteljske rastline; pehA; multipleks PCR; VCF3/VCR3

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720//aas.2014.103.2.16

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