Genetic studies of soybean [Glycine max (L.) Merr.] response to seed storage stress factors

Gbemisola Oluseun OKUNLOLA, Emmanuel IDEHEN, Johnson Adedayo ADETUMBI, Christopher ALAKE, Solomon Tayo AKINYOSOYE, Oluwafemi Daniel AMUSA


Soybean [Glycine max (L.) Merr.] responded differently to storage stress factors. This research work aimed at assessing genetic potentials of fifteen soybean varieties to storage stress via accelerated aging technique and grouping them based on their levels of tolerance to storage stress using simple sequence repeat (SSR). Tolerance of the seed of 15 soybean varieties to storage stress was assessed by subjecting them to accelerated aging for 0, 6, 12, and 24 hours at 42 °C temperature and 100 % relative humidity (RH), after which their quality was assessed to determine their tolerance to storage stress. Same varieties were also stored for 6 months under ambient environment at 65 ± 5 % R.H and 25 ± 2 °C and they were genotyped with 19 simple sequence repeat (SSR) markers. The varieties were grouped on the basis of their levels of tolerance to storage stress. The principal components analysis (PCA) showed that germination rate index (GRI) and germination index (GI) were the major indices responsible for the significant variation in the seedling vigor characters. TGX1835-10E and TGX1448-2E were identified as varieties with good storage ability and therefore recommended for storage improvement in soybean breeding programs. Six SSR markers (Satt 565, Satt 175, Satt 281, Satt 600, Satt 160 and Satt 281) were identified as candidate markers for detection of alleles for tolerance to storage stress.


soybean; seed accelerated aging; principal components analysis (PCA); dendrogram; SSR markers; tolerance

Full Text:



Abbey, T. K. Essiah, J.W., Alhassan, A., Fometu, E., Ameyibor, K., and Wiredu, M. B. (2001). Integrated Science for Senior Secondary School. Unimax MacMillan Ltd. Ring Road South industrial Area Accra-North Ghana. Pp 177-178.

Adebisi, M. A., I. O. Daniel, I. O., M. O. Ajala, M. O. (2004a). Storage life of soybean seed after seed dressing. Journal of Tropical Agriculture, 42, 3-7.

Adebisi, M.A., Ajala, M.O., Ariyo. O.J. (2004b). Genetic studies on seed quality in sesame. Proceedings of the 29th Annual Conference of the Genetics Society of Nigeria. Pp. 209-212.

Adebisi, M.A., Okelola, F.S., Alake, C.O., Ayo-Vaughan, M.A., Ajala, M.O. (2010). Interrelationship between seed vigour traits and field performance in new rice for Africa (Nerica) genotypes (Oryza sativa L.). Journal of Agricultural Science and Environment, 10(2), 15-24.

Akande, S. R.,Olakojo, S. A., Ajayi, S. A., Owolade, O.F., Adetumbi, J. A., Adeniyan, O. N., Ogunbodede, B. A. (2012). Planting date effects on cowpea seed yield and quality at southern guinea Savanna of Nigeria. Seed Technology, 34, 79-88.

Badu-Apraku, B., Yallou, C.G., Obeng-Antwi, K., Alidu, H., Talabi, A.O., Annor, B., Oyekunle, M., Akaogu. I.C. and Aderounmu, M. (2017).Yield gains in extra-early maize cultivars of three breeding eras under multiple environments. Agronomy Journal, 109(2), 418.

Baskin, C. C., Delouche, J.C. (1973). Accelerated aging techniques for predicting the relative storability of seed lots. Seed Science & Technology, 14, 427-452.

Bhatia V. S. (1996). Seed longevity as affected by field weathering and its association with seed coat and pod characters in soybean. Seed Research, 24, 82-87.

Brown, J., and P. Caligari, (2008). An introductionto plant breeding. Blackwell Publishing Ltd, Oxford, UK.

Camara, Y. (2000). Profitability of cassava production system in West Africa: A comparative analysis (Cote. D’ Ivore, Ghana and Nigeria), Ph. D. Thesis, Michigan State University, East.

Clerkx, E.J.M., El-Lithy, M.E., Vierling, E., Ruys, G.J., Blankestijin-De Vries, H., Groot S.P.C., Vreugdenhil, D., Koornnee, F.M. (2004). Analysis of natural allelic variation of Arabidopsis seed germination and seedlongevity traits between the accessions Landsbergerecta and Shakdara, using a new recombinant inbred line population. Plant Physiology, 135, 432–443.

DargahiH.,Tanya, P., Srinives, P. (2014). Mapping of the genomic regions controlling seed storability in soybean (Glycine max (L.) Merill.). Journal of Genetics, 93, 365–370.

Demir, I., Mavi, K. (2008). Controlled deterioration and accelerated aging tests to estimate the relative storagepotential of Cucurbit seed lots. Horticultural science, 43, 1544-1548.

Dugje, I.Y., Omoigui, L.O., Ekeleme, F., Bandyopadhyay, R., Lava Kumar, P., Kamara, A.Y. (2009). Farmers’ Guide to Soybean Production in Northern Nigeria. International Institute of Tropical Agriculture, Ibadan, Nigeria. 21 pp

Flajšman, M., Šantavec, I., Kolmanič, A., Košmelj, K., & Kocjan-Ačko, D. (2019). Agronomic performance and stability of seed, protein and oil yields of seven soybean cultivars determined in field experiments in Slovenia. Genetika, 51(1), 31-46.

Hammer, D.A., Harper, T., Ryan, P.D. (2001). PAST: Palaeontological Statistics, version 2.17 software package for education and data analysis. Palaeontologia electronica, 4(1), 9 pp.

Hartman, G.L., West, E.D., Herman T. K. (2011). Crops that feed the World 2. Soybean worldwide production, use, and constraints caused by pathogens and pests. Food Security, 3, 5–17.

Hedley, C.L. ed. (2001). Carbohydrates in Grain Legume Seeds: Improving Nutritional Quality and Agronomic Characteristics. CABI Publishing: New York.

ISTA, (1996). Handbook of Vigor Test Methods (2ndEdn), International Seed Testing Association, Zurich,Switzerland, pp 28-37.

Jagadish, H.A, Kumar, M.B, Talukdar, A., Lal1, S.K., Dadlani, M. (2013). Molecular characterization and identification of candidate markers for seed longevity in soybean [Glycine max (L.) Merill]. Indian Journal of Genetics,73(1), 64-71.

Kader, M.A. (2005). A comparison of seed germination calculation formulae and the associated interpretation of resulting data. Journal and Proceedings of the Royal Society of New South Wale, 138, 65-75.

Kehinde, T.O., Ajala, M.O., Daniel, I.O., Oyelakin, O.O. (2013). Physiological and genetic integrity of amaranth (Amaranthus spp.) seed during storage. International Journal of Plant Breeding and Seed Genetic, 7(1), 35-46.

Liu, K. and Muse, S.V. (2005). PowerMaker: integrated analysis environment for genetic marker data. Bioinformatics, 21(3), 2128-2129.

Matus, I., Gonzaléz, M.I., Pozo, A. (1999). An evaluation of phenotypic variation in a Chilean collection of garlic (Allium sativum L.) clones using multivariate analysis. Plant Genetic Resources Newsletter, 117, 31-36.

Nkang, A., Umho, E. O. (1996). Six month storability of five soybean cultivars as influenced by stage of harvest, storage temperature and relative humidity. Seed Science and Technolology, 25, 93-99.

Olisa, B.S., Ajayi, S.A., Akande, S.R. (2010). Physiological quality of seeds of promising African yam bean (Sphenomstylis stenocarpa (Hoechst. Ex A. Rich)Harms) and pigeon pea(Cajanus cajan (L.) Mill sp.) landraces. Research Journal of Seed Sciences, 3(2), 93-101.

Scott, S.J., Jones, R.A., Williams, W.A. (1984). Review of data analysis methods for seed germination. Crop Science, 24, 1192-l 199.

Singh, R. K., Raipuria, R. K., Bhatia, V. S., Rani, A., Pushpendra, T., Husain, S. M.,Satyavathi, C. T., Chauhan, G. S., Mohapatra, T. (2008). Identification of SSR markers associated with seed coat permeability and electrolyte leaching in soybean. Physiology and Molecular Biology of Plants, 14, 173-177.

Statistical Tool for Agricultural Research (STAR, Version: 2.0.1, 2013 - 2020). Rice Research Institute (IRRI).

Verma, D.P.S., Shoemaker, R.C. eds. (1996). Soybean Genetics, Molecular Biology and Biotechnology. CAB International: Wallingford.

Yuesheng, W., Jianbing, Q., Junyi, G., Guangyuang, H. (2006). Classification and characteristic of maturity groups of Chinese landraces of Soybean [Glycine max (L.) Merr.]. Genetic Resources and Crop Evolution, 53(4), 803–809.



  • There are currently no refbacks.

Copyright (c) 2020 Gbemisola Oluseun Okunlola

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.


eISSN 1854-1941