Induction of defence related enzymes and biocontrol efficacy of Trichoderma harzianum in tomato plants infected with Fusarium oxysporum and Fusarium solani

Tavga Sulaiman RASHID, Sirwa Anwar QADIR, Hayman Kakakhan AWLA

Abstract


Fusarium wilt of tomato plants caused by Fusarium oxysporum Schlecht. emend. Snyder & Hansen and Fusarium solani (Mart.) Sacc. are serious problem limiting tomato production worldwide. Biological control has emerged as one of the most promising alternatives to chemical fungicides. The biological control capability of a T. harzianum isolate against F. solani and F. oxysporum has been investigated. It inhibited colony growth of two Fusarium species by more than 80 % in dual culture tests. Results of greenhouse experiments revealed that disease severity in the tomato plants co-inoculated with T. harzianum was significantly lower than plants only infected with the Fusarium pathogens. Tomato plants inoculated with the antagonistic T. harzianum isolate, showed enhanced peroxidase and polyphenol oxidase activities in greenhouse experiments and increased resistance to F. solani and F. oxysporum. The T. harzianum isolate indirectly affected the Fusarium pathogens by enhancing plant defence.


Keywords


fungal pathogens; antagonistic fungus; peroxidase; polyphenol oxidase; plant growth and biological control

Full Text:

PDF

References


Abd-El-Khair H, Khalifa R K M, Haggag K H. (2010). Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. American Journal of Science, 6(9), 486-497.

Abo-Elyousr K A, Hashem M, Ali E H. (2009). Integrated control of cotton root rot disease by mixing fungal biocontrol agents and resistance inducers. Crop Protection, 28(4), 295-301. https://doi.org/10.1016/j.cropro.2008.11.004

Alwathnani H A, Perveen K, Tahmaz R, Alhaqbani S. (2012). Evaluation of biological control potential of locally isolated antagonist fungi against Fusarium oxysporum under in vitro and pot conditions. African Journal of Microbiology Research, 6(2), 312-319. https://doi.org/10.5897/AJMR11.1367

Arora R, Wisniewski M E. (1994). Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica [L.] Batsch)(II. A 60-kilodalton bark protein in cold-acclimated tissues of peach is heat stable and related to the dehydrin family of proteins). Plant Physiology, 105(1), 95-101. https://doi.org/10.1104/pp.105.1.95

Boyaci F, Unlu A, Abak K. (2010). August. Screening for resistance to Fusarium wilt of some cultivated eggplants and wild Solanum accessions. In XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New 935 (pp. 23-27). https://doi.org/10.17660/ActaHortic.2012.935.2

Chandra A, Bhatt R K. (1998). Biochemical and physiological response to salicylic acid in relation to the systemic acquired resistance. Photosynthesis, 35, 255–258. https://doi.org/10.1023/A:1006966908357

Chao W l, Nelson E B, Harman G E, Hoch H C. (1986). Colonization of the rhizosphere by biological control agents applied to seeds. Phytopathology, 76, 60-65. https://doi.org/10.1094/Phyto-76-60

Christopher D J, Raj T S, Rani S U, Udhayakumar R. (2010). Role of defense enzymes activity in tomato as induced by Trichoderma virens against Fusarium wilt caused by Fusarium oxysporum f sp. lycopersici. Journal of Biopesticides, 3(1), 158-162.

de los Santos-Villalobos S, Barrera-Galicia G C, Miranda-Salcedo M A, Peña-Cabriales J J. (2012). Burkholderia cepacia XXVI siderophore with biocontrol capacity against Colletotrichum gloeosporioides. World Journal of Microbiology and Biotechnology, 28(8), 2615-2623. https://doi.org/10.1007/s11274-012-1071-9

FAOSTAT (2017). Production – Crops – Area harvested/ Production quantity – Tomatoes – 2014, FAO Statistics online database, Food and Agriculture Organization, Rome, www.fao.org/faostat/en (accessed 22 Sept. 2017).

Henry A, Kleinman P J, Lynch J P. (2009). Phosphorus runoff from a phosphorus deficient soil under common bean (Phaseolus vulgaris L.) and soybean (Glycine max L.) genotypes with contrasting root architecture. Plant and Soil, 317(1-2), 1-16. https://doi.org/10.1007/s11104-008-9784-0

Herrera-Téllez V I, Cruz-Olmedo A K, Plasencia J, Gavilanes-Ruíz M, Arce-Cervantes O, Hernández-León S, Saucedo-García M. (2019). The protective eEffect of Trichoderma asperellum on tomato plants against Fusarium oxysporum and Botrytis cinerea diseases involves inhibition of reactive oxygen species production. International Journal of Molecular Sciences, 20(8), 2007. https://doi.org/10.3390/ijms20082007

Houssien A A, Ahmed S M, Ismail A. A. (2010). Activation of tomato plant defense response against Fusarium wilt disease using Trichoderma harzianum and salicylic acid under greenhouse conditions. Research Journal of Agriculture and Biological Sciences, 6(3), 328-338.

Kloepper J W, Wei G, Tuzun S. (1992). Rhizosphere population dynamics and internal colonization of cucumber by plant growth-promoting rhizobacteria which induce systemic resistance to Colletotrichum orbiculare. In Biological control of plant diseases (pp. 185-191). Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9468-7_24

Mahato S, Bhuju S, Shrestha J. (2018). Effect of Trichoderma viride as biofertilizer on growth and yield of wheat. Malays Journal of Sustainable Agriculture, 2(2), 1-5. https://doi.org/10.26480/mjsa.02.2018.01.05

Morkunas I, Gmerek J. (2007). The possible involvement of peroxidase in defense of yellow lupin embryos axes against Fusarium oxysporum. Journal of Plant Physiology, 164(6), 497–506. https://doi.org/10.1016/j.jplph.2005.11.005

Malandrakis A, Daskalaki E R, Skiada V, Papadopoulou K K, Kavroulakis N. (2018). A Fusarium solani endophyte vs fungicides: Compatibility in a Fusarium oxysporum f. sp. radicis-lycopersici–tomato pathosystem. Fungal Biology, 122(12), 1215-1221. https://doi.org/10.1016/j.funbio.2018.10.003

Morsy E M, Abdel-Kawi K A, Khalil M N A. (2009). Efficiency of Trichoderma viride and Bacillus subtilis as biocontrol agents gainst Fusarium solani on tomato plants. Egyptian Journal of Phytopathology, 37, 47-57.

Ojha S, Chatterjee N. (2012). Induction of resistance in tomato plants against Fusarium oxysporum f. sp. lycopersici mediated through salicylic acid and Trichoderma harzianum. Journal of Plant Protection Research, 52(2), 220-225. https://doi.org/10.2478/v10045-012-0034-3

Otadoh J, Okoth S, Ochanda J, Kahindi J. (2010). Assessment of Trichoderma isolates for virulence efficacy on Fusarium oxysporum f. sp. phaseoli. Tropical and Subtropical Agroecosystems, 13(1), 99-107.

Perello A, Monaco C, Simon M R, Sisterna M, Dalbello G. (2003). Biocontrol efficacy of Trichoderma isolates for tar spot of wheat in Argentina. Crop Protection, 22(7), 1099–1106. https://doi.org/10.1016/S0261-2194(03)00143-1

Portal N, Soler A, Alphonsine P A M, Borras‐Hidalgo O, Portieles R, Peña‐Rodriguez L M, Yanes E, Herrera L, Solano J, Ribadeneira C, Walton J D. (2018). Nonspecific toxins as components of a host‐specific culture filtrate from Fusarium oxysporum f. sp. cubense race 1. Plant Pathology, 67(2), 467-476. https://doi.org/10.1111/ppa.12736

Pradeep T, Jambhale N D. (2002). Relationship between phenolics, polyphenol oxidase and peroxidases and resistance to powdery mildew in Zizhyphus. Indian Phytopathology, 55(2), 195-196.

Rahman M A, Begum M F, Alam M F. (2009). Screening of Trichoderma isolates as a biological control agent against Ceratocystis paradoxa causing pineapple disease of sugarcane. Mycobiology, 37(4), 277-285. https://doi.org/10.4489/MYCO.2009.37.4.277

Ramdoss N. (1991). Studies on the epidemiology, pathophysiology and management of Thanjavur wilt of coconut (Doctoral dissertation, Tamil Nadu Agricultural University; Coimbatore).

Rashid, T. S., Sijam, K., Awla, H. K., Saud, H. M., & Kadir, J. (2016). Pathogenicity assay and molecular identification of fungi and bacteria associated with diseases of tomato in Malaysia. American Journal of Plant Sciences, 7(6), 949-957. https://doi.org/10.4236/ajps.2016.76090

Silva R N, Monteiro V N, Steindorff A S, Gomes E V, Noronha E F, Ulhoa C J. (2019). Trichoderma/pathogen/plant interaction in pre-harvest food security. Fungal Biology, 123(8), 565-583. https://doi.org/10.1016/j.funbio.2019.06.010

Sreedevi B, Devi M C, Saigopal D V R. (2011). Induction of defense enzymes in Trichoderma harzianum treated groundnut plants against Macrophomina phaseolina. Journal of Biological Control, 25(1), 33-39.

Verma M, Brar S K, Tyagi R D, Surampalli R Y, Valero J R. (2007). Antagonistic fungi, Trichoderma spp.: panoply of biological control. Biochemical Engineering Journal, 37(1), 1-20. https://doi.org/10.1016/j.bej.2007.05.012

Vidhyasekaran P. (2004). Concise encyclopedia of plant pathology. CRC Press. https://doi.org/10.1201/9781482277951




DOI: http://dx.doi.org/10.14720/aas.2021.117.1.1622

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Tavga Sulaiman Rashid, Sirwa Anwar Qadir, Hayman Awla

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY-NC-ND 4.0 License.

                            


eISSN 1854-1941