An efficient protocol for in vitro regeneration from the nodal explants of Withania coagulans (Stocks) Dunal: a valuable medicinal herb

Pari DEHVARI-NAGAN, Hosein ABBASPOUR, Mohammad Hasan ASARE, Sara SAADATMAND

Abstract


In order to develop a protocol for the effective micropropagation of the important medicinal plant Withania coagulans (Stocks) Dunal, the effects of different concentrations and combinations of growth regulators on the nodal explants in two independent experiments were investigated. For shooting, a MS medium fortified with different concentrations and combinations of IBA (0.01, 0.1 and 0.5 mg l-1), BA (0.5, 1 and 2 mg l-1), Kin (0.5 and 1 mg l-1), PG (0.5 mg l-1) and GA (0.5 mg l-1) was used and the highest shooting response, shoot number and shoot length were obtained in the MS + IBA (0.01 mg l-1) + BA (0.5 mg l-1) + PG (0.5 mg l-1) + GA (0.5 mg l-1) treatment. In the second experiment, the effect of MS supplemented with different combinations and concentrations of IBA (0.1, 0.5, 1 and 2 mg l-1), NAA (0.1 and 1 mg l-1) and PG (1 mg l-1) on rooting of the nodal explants was investigated, which showed that the highest rooting response (%) was observed in the MS fortified with NAA (0.1 mg l-1), NAA (1 mg l-1), NAA (0.1 mg l-1) + PG (1 mg l-1), and NAA (1 mg l-1) + PG (1 mg l-1) treatments, as well as the highest number of roots at NAA (0.1 mg l-1) and the highest root length at IBA (1 mg l-1). Our findings highlight a complete micropropagation method for W. coagulans from the nodal explant that can make a significant contribution to the development of W. coagulans material for medical applications.


Keywords


Withania coagulans; micropropagation; in vitro; nodal explant; phloroglucinol

Full Text:

PDF

References


Ahmed, M. B., Salahin, M., Karim, R., Razvy, M. A., Hannan, M. M., Sultana, R., Hossain, M., & Islam, R. (2007a). An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni.) in Bangladesh. American-Eurasian Journal of Scientific Research, 2(2), 121-125.

Ahmed, S., Kabir, A. H., Ahmed, M. B., Razvy, M. A., & Ganesan, S. (2007b). Development of rapid micropropagation method of Aloe vera L. Sjemenarstvo, 24, 121–128.

Chen, L. X., He, H., & Qui, F. (2011). Natural withanolides: an overview. Nature Product Reports, 28, 705–740. https://doi.org/10.1039/c0np00045k

Gerami, M., Ghorbani, A., & Karimi, S. (2018). Role of salicylic acid pretreatment in alleviating cadmium-induced toxicity in Salvia officinalis L. Iranian Journal of Plant Biology, 10(1), 81-95.

Ghasemi-Omran VO, Ghorbani A, Sajjadi-Otaghsara SA (2021) Melatonin alleviates NaCl-induced damage by regulating ionic homeostasis, antioxidant system, redox homeostasis, and expression of steviol glycosides-related biosynthetic genes in in vitro cultured Stevia rebaudiana Bertoni. In Vitro Cell Dev Biol.-Plant. https://doi.org/10.1007/s11627-021-10161-9

Ghorbani, A., Ghasemi Omran, V. O., Razavi, S. M., Pirdashti, H., & Ranjbar, M. (2019). Piriformospora indica confers salinity tolerance on tomato (Lycopersicon esculentum Mill.) through amelioration of nutrient accumulation, K+/Na+ homeostasis and water status. Plant Cell Reports, 38, 1151–1163. https://doi.org/10.1007/s00299-019-02434-w

Ghorbani, A., Razavi, S. M., Ghasemi Omran, V. O., & Pirdashti, H. (2018). Piriformospora indica inoculation alleviates the adverse effect of NaCl stress on growth, gas exchange and chlorophyll fluorescence in tomato (Solanum lycopersicum L.). Plant Biology, 20(4), 729-736. https://doi.org/10.1111/plb.12717

Gilani, S. A., Kikuchi, A., & Watanabe, K. N. (2009). Genetic variation within and among fragmented populations of endangered medicinal plant, Withania coagulans (Solanaceae) from Pakistan and its implications for conservation. African Journal of Biotechnology, 8, 2948–2958.

Haq, I., Youn, U. J., Chai, X., Park, E. J., Kondratyuk, T. P., Simmons, C. J., Borris, R. P., Mirza, B., Pezzuto, J. M., & Chang, L. C., (2013). Biologically active withanolides from Withania coagulans. Journal of Natural Products, 76, 22–28. https://doi.org/10.1021/np300534x

Ibanez, M. R., & Amo-Marc, J. B. (1998). Promotion by phloroglucinol of micropropagation of Minuartia valentina, an endangered and endemic Spanish plant. Plant Growth Regulators, 26, 49-56. https://doi.org/10.1023/A:1006050122173

Jain, R., Sinha, A., Jain, D., Kachhwaha, S., & Kothari, S. L. (2010). Adevntitious shoot regeneration and in vitro biosynthesis of steroidal lactones in Withania Coagulans (Stocks) Dunal. Plant Cell, Tissue and Organ Culture, 105, 135-140 https://doi.org/10.1007/s11240-010-9840-3

Maurya, R., & Akanksha, J. (2010). Chemistry and pharmacology of Withania coagulans: an ayurvedic remedy. Journal of Pharmacy and Pharmacology, 62, 153–160. https://doi.org/10.1211/jpp.62.02.0001

Rathore, M. S., Mastan, S. G., Yadav, P., Bhatt, V. D., Shekhawat, N. S., & Chikara, J. (2016). Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers. South African Journal of Botany, 102, 12–17. https://doi.org/10.1016/j.sajb.2015.08.003

Rathore, M. S., Shekhawat, S., Kaur, G., Singh, R. P., & Shekhawat, N. S. (2012). Micropropagation of vegetable rennet (Withania coagulans [Stocks] Dunal)—a critically endangered medicinal plant. Journal of Sustainable Forestry, 31, 727–746. https://doi.org/10.1080/10549811.2012.706533

Saritha, K. V., & Naidu, C. V. (2007). In vitro flowering of Withania somnifera Dunal. -An important antitumor medicinal plant. Plant Science, 172, 847-851. https://doi.org/10.1016/j.plantsci.2006.12.016

Sivansean, I., & Murugesan, K. (2008). An efficient regeneration from nodal explants of Withania somnifera Dunal. Asian Journal of Plant Sciences, 7(6), 551- 556. https://doi.org/10.3923/ajps.2008.551.556

Valizadeh, J., & Valizadeh, M. (2009). In vitro callus induction and plant regeneration from Withania coagulans: A valuable medicinal plant. Pakistan Journal of Biological Sciences, 12(21), 1415-1419. https://doi.org/10.3923/pjbs.2009.1415.1419

Valizadeh, J., & Valizadeh, M. (2011). Development of efficient micropropagation protocol for Withania coagulans (Stocks) Dunal. African Journal of Biotechnology, 10(39), 7611-7616




DOI: http://dx.doi.org/10.14720/aas.2021.117.2.1754

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Pari DEHVARI-NAGAN, Hosein Abbaspour, Mohammad Hasan ASARE, Sara SAADATMAND

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.

                           


eISSN 1854-1941