Comparison of shoot and root regeneration of miniature potted rose (Rosa x hybrida L.) and Damask rose (R. damascena Mill.) in microculture system

Farkhondeh REZANEJAD, Somayeh ABDIRAD, Moslem ABARIAN

Abstract


Miniature potted rose and Damask rose are important commercial plant cultivars in ornamental horticulture. Root suckers are common rose propagation method, but it is slow and seasonally dependent. In this survey, the propagation of nodal explants of these two species was studied through in vitro regeneration system. 16 and 24 different media were used for study of shoot and root regeneration respectively. The axillary buds were sprouted earlier in miniature rose than R. damascena. Shoot induction and proliferation (shoot ramification and growth) were observed 5 and 17 days after planting in miniature rose and 16 and 38 days in R. damascena respectively. The highest shoot proliferation obtained in media 3 and 7 in miniature rose, and medium 16 for R. damascena. These three media were recorded as optimal media with 100 % shoot proliferation. In these media, root initiation and growth of miniature rose (respectively after 78 and 92 days) was earlier than Damask rose (respectively 125 and 138 days). The successful rooting occurred in three and two media for miniature and Damask rose respectively. Rooting frequency was higher in the half strength MS liquid media than the others. Thus, cultivar potted rose as a modern species is propagated easier than old rose (R. damascena).


Keywords


micropropagation; proliferation; rooting rate

Full Text:

PDF

References


Abidin, N., and Metali, F. (2015). Effects of different types and concentrations of auxins on juvenile stem cuttings for propagation of potential medicinal Dillenia suffruticosa martelli shrub. Research Journal of Botany, 10(3), 73-87. https://doi.org/10.3923/rjb.2015.73.87

Ahmadian, E., Lolaei, A., Mobasheri, S., Bemana, R. (2013). Investigation of importance parameters of plant tissue (review). International Journal of Agriculture and Crop Sciences, 5(8), 900-905.

Alsemaan, T. (2013). Micro-propagation of Damask Rose (Rosa damascena Mill.) cv. Almarah. International Journal of Agricultural Research, 8(4), 172-177. https://doi.org/10.3923/ijar.2013.172.177

Asadi, A.A., Vedadi, C., Rahimi, M., Naserian, B. (2009). Effect of plant growth hormones on root and shoot regeneration in rose (Morrasia) under in-vitro conditions. Bioscience Research, 6(1), 40-45.

Badzian, T., Hennen, G.R., Fotyma-Kern, J. (1991). In vitro rooting of clonal propagated miniature rose cultivars. In International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement, 289, 329-330. https://doi.org/10.17660/ActaHortic.1991.289.81

Bidabadi, S.S., Jain, S. M. (2020). Cellular, molecular, and physiological aspects of in vitro plant regeneration. Plants, 9(6), 702. https://doi.org/10.3390/plants9060702

Brailko, V.A., Plugatar, S.A., Pilipchuk, T.I., Plugatar, Y.V., Mitrofanova, I.V. (2017). Morphological and physiological features of the miniature rose cultivar Risen Shine under long time culture in vitro and in vivo. In VII International Symposium on Production and Establishment of Micropropagated Plants, 1224, 139-144. https://doi.org/10.17660/ActaHortic.2018.1224.19

Cai, Z., Jing, X., Tian, X., Jiang, J., Liu, F., Wang, X. (2015). Direct and indirect in vitro plant regeneration and the effect of brassinolide on callus differentiation of Populus euphratica. South African Journal of Botany, 97, 143-148. https://doi.org/10.1016/j.sajb.2015.01.006

Davoudi Pahnekolayi, M., Samiei, L., Tehranifar, A., Shoor, M. (2015). The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.). Journal of Plant Molecular Breeding, 3(1), 61-71.

Edwin, F.G., Paul, D.Sh. (1984). Plant Propagation by Tissue Culture. Handbook and Directory of Comerical Laboratories. Exegetics Ltd., Eversley, Basingstoke, Hants, UK.

Ginova, A., Tsvetkov, I., kondakova, V. (2012). Rosa damascene Mill. An overview for evaluation of propagation methods. Bulgarian Journal of Agricultural Science, 18, 545-556.

Gubis, J., Lajchova, Z., Farago, J. Jurekova, Z. (2003). Effect of genotype and explant type on shoot regeneration in tomato (Lycopersicon esculentum) in vitro. Czech Journal of Genetics and Plant Breeding, 39(1), 9-14. https://doi.org/10.17221/3715-CJGPB

Hobbie, L., McGovern, M., Hurwitz, L.R., Pierro, A., Liu, N.Y., Bandyopadhyay, A., Estelle, M. (2000). The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development. Development, 127(1), 23-32. https://doi.org/10.1242/dev.127.1.23

Jabbarzadeh, Z., Khosh-Khui, M. (2005). Factors affecting tissue culture of Damask rose (Rosa damascena Mill.). Scientia Horticulturae, 105(4), 475-482. https://doi.org/10.1016/j.scienta.2005.02.014

Kadhimi, A.A., Alhasnawi, A.N., Mohamad, A., Yusoff, W.M.W., Zain, C. (2014). Tissue culture and some of the factors affecting them and the micropropagation of strawberry. Life Science Journal, 11(8), 484-493.

Khaleghi, A., Khadivi, A. (2020). Morphological characterization of Damask rose (Rosa× damascena) germplasm to select superior accessions. Genetic Resources and Crop Evolution, 67(8), 1981-1997. https://doi.org/10.1007/s10722-020-00954-z

Khaskheli, A.J., Khaskheli, M.I., Khaskheli, M.A., Shar, T., Ahmad, W., Lighari, U.A., Khaskheli, M.A., Khaskheli, A.A. and Makan, F.H. (2018). Proliferation, multiplication and improvement of micro-propagation system for mass clonal production of rose through shoot tip culture. American Journal of Plant Sciences, 9, 296-310. https://doi.org/10.4236/ajps.2018.92024

Kim C.K., Oh J.Y., Jee S.O., Chung J.D. (2003). In vitro micropropagation of Rosa hybrid L. Plant Biotechnology, 5, 115-119.

Kirichenko, E.B., Kuz-Mina, T.A., Kataeva, N.V. (1991). Factors in optimizing the multiplication of ornamental and essential oil roses in vitro. Byulleten-Glavnogo-Botanicheskogo Sada, 159, 61–67.

Ma, Y., Byrne, D. H., Chen, J. (1996). Propagation of rose species in vitro. In vitro Cellular and Developmental Biology - Plant, 32(2), 103-108. https://doi.org/10.1007/BF02823139

Mahmoudi Noodezh H.M., Moieni, A., Baghizadeh, A. (2012). In vitro propagation of the Damask rose (Rosa damascena Mill.), In Vitro Cellular and Developmental Biology – Plant, 48(5), 530-538. https://doi.org/10.1007/s11627-012-9454-z

Muiruri, S.N., Mweu, C.M. and Nyende, B.A. (2011). Micropropagation protocols using nodal explants of selected rose (Rosa hybrida) cultivars. African Journal of Horticultural Science, 4, 60-65.

Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nikbakht, A., Kafi, M., Mirmasoudi, M., Babalar, M. (2005). Micropropagation of damask rose (Rosa damascena Mill.) cvs Azaran and Ghamsar. International Journal of Agriculture and Biology, 7(4), 535-538.

Ozkan, G., Sagdic, O., Baydar, N.G., Baydar, H. (2004). Note: Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Science and Technology International, 10(4), 277-281. https://doi.org/10.1177/1082013204045882

Pati, P.K., Rath, S.P., Sharma, M., Sood, A., Ahuja, P.S. (2006). In vitro propagation of rose- a review. Biotechnology advances, 24(1), 94-114. https://doi.org/10.1016/j.biotechadv.2005.07.001

Pati, P.K., Kaur, N., Sharma, M. and Ahuja, P.S. (2010). In vitro propagation of rose. In Protocols for in vitro Propagation of Ornamental Plants, 163-176. https://doi.org/10.1007/978-1-60327-114-1_16

Pierik, R.L.M. (1991). Horticulture new technologies and applications proceeding of the international seminar on new frontiers in horticulture. Current Plant Science and Biotechnology in Agriculture, 12, 141–153. https://doi.org/10.1007/978-94-011-3176-6_23

Tarrahi, R., Rezanejad, F. (2013). Callogenesis and production of anthocyanin and chlorophyll in callus cultures of vegetative and floral explants in Rosa gallica and R. hybrida (Rosaceae). Turkish Journal of Botany, 37(6), 1145-1154. https://doi.org/10.3906/bot-1205-42

Thi, K.O., Khai, A.A., Lwin, K.M. (2008). Micropropagation of Rose (Rosa hubrida spp.) by in vitro culture technique. GMSARN International Conference on Sustainable Development: Issues and Prospects forthe GMS, 12-14.

Tibkwang, A., Junkasiraporn, S., Chotikadachanarong, K. (2018). Effects of Cytokinnin and Sucrose on Tissue Culture of Rosa chinensis. Burapha Science Journal, 23(2), 712-721.

Younis, A., Riaz, A., Javaid, F., Ahsan, M., Tariq, U., Aslam, S. Majeed, N. (2015). Influence of various growing substrates on growth and flowering of potted miniature rose cultivar “Baby Boomer”. Specialty Journal of Agricultural Sciences, 1(2), 28-33.




DOI: http://dx.doi.org/10.14720/aas.2023.119.1.2380

Refbacks

  • There are currently no refbacks.


Copyright (c) 2023 Farkhondeh REZANEJAD, Somayeh ABDIRAD, Moslem ABARIAN

 

Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.

                           


eISSN 1854-1941