Enhance the phytoremediation efficiency of Echinochloa colona (L.) Link for Pb-contaminated soil by phosphorus solubilizing bacteria

Quang Trung DO

Abstract


A promising solution for phytoremediation of metal-contaminated soils is to use plants in combination with phosphate-solubilizing bacteria (PSB). In this study, we subjected to isolate PSB from paddy soil and investigate their ability in improving the phytoremediation of lead (Pb2+) by a weed plant (Echinochloa colona (L.) Link) as well as in promoting the growth of E. colona under Pb stress condition. Total 06 PSB (labeled from TB01 to TB06) were isolated and the TB04 showed the strongest phosphate-solubilizing activity with the highest values of phosphorus solubilization index (PSI = 7.13) obtained from Ca3(PO4)2. Especially, the phosphorus solubilizing ability of the TB04 strain was not affected by the high Pb2+concentration. The TB04 strain was identified as Pseudomonas putida Trevisan, 1889 (accession number FJ976601.1). Furthermore, E. colona inoculated with TB04 strain significantly increased the phytoremediation efficiency of Pb from Pb-contaminated soil and the growth was enhanced clearly. These results suggest that the TB04 strain could potentially use as an inoculant in combination with E. colona to construct novel constructed wetlands for phytoremediation of metal-contaminated soil.


Keywords


lead immobilization; Pseudomonas putida; soil fertility; phytoremediation; metal-contaminated soil

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References


Aransiola, S.A., Ijah, U.J.J., Abioye, O.P. and Bala, J.D. (2019). Microbial-aided phytoremediation of heavy metals contaminated soil: a review. European Journal of Biological Research, 9(2), 104-125.

Adhikari, A., Lee, K.E., Khan, M.A., Kang, S.M., Adhikari, B., Imran, M., Jan, R., Kim, K.M. and Lee, I.J. (2020). Effect of silicate and phosphate solubilizing rhizobacterium Enterobacter ludwigii GAK2 on Oryza sativa L. under cadmium stress. Journal of Microbiology and Biotechnollogy, 30(1), 118-126. https://doi.org/10.4014/jmb.1906.06010

Fankem, H., Nwaga, D., Deubel, A., Dieng, L., Merbach, W. and Etoa, F.X. (2006). Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon. African Journal of Biotechnology, 5(24), 2450-2460.

Himani, S. and Reddy, M.S. (2011). Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils. European Journal of Soil Biology, 47(1), 30–34. https://doi.org/10.1016/j.ejsobi.2010.10.005

Jones, J.B. and Case, V.W. (1990). Sampling, handling, and analyzing plant tissue samples. In Westerman RL (ed.). Soil Testing and Plant Analysis. Soil Science Society of America, Inc., Madison, WI, pp. 389-447. https://doi.org/10.2136/sssabookser3.3ed.c15

Kumar, A. and Rai, L.C. (2015). Proteomic and biochemical basis for enhanced growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium. Microbiology Research, 170, 195-204. https://doi.org/10.1016/j.micres.2014.06.006

Katiyar, V. and Goel, R. (2003). Solubilization of inorganic phosphate and plant growth promotion by cold tolerant mutants of Pseudomonas fluorescens. Microbiology Research, 158(2), 163-168. https://doi.org/10.1078/0944-5013-00188

Liu, Z., Li, Y.C., Zhang, S., Fu, Y., Fan, X., Patel, J. S. and Zhang, M, (2015). Characterization of phosphate-solubilizing bacteria isolated from calcareous soils. Applied Soil Ecology, 96, 217–224. https://doi.org/10.1016/j.apsoil.2015.08.003

Lin, M., Jin, M., Xu, K., He, L. and Cheng, D. (2018). Phosphate-solubilizing bacteria improve the phytoremediation efficiency of Wedelia trilobata for Cu-contaminated soil. International Journal of Phytoremediation, 20(8), 813-822. https://doi.org/10.1080/15226514.2018.1438351

Marra, L.M., de Oliveira-Longatti, S.L., Cláudio R.F.S.S., Fábio L.O. and de Souza Moreira F.M. (2019). The amount of phosphate solubilization depends on the strain, C-source, organic acids and type of phosphate. Geomicrobiology Journal, 36(3), 232-242. https://doi.org/10.1080/01490451.2018.1542469

Nakhro, N. and Dkhar, M.S. (2010). Impact of organic and inorganic fertilizers on microbial population and biomass carbon in paddy field soil. Journal of Agronomy, 9(3), 102-110. https://doi.org/10.3923/ja.2010.102.110

Noble, A., Banakuna, F., Tanee, G. and Osuji, J. (2018). The effect of ripe plantain peels waste on the phytoextraction of Pb and Cd by Echinochloa colona (L.) Link. International Journal of Natural Resource Ecology and Management, 3(1), 19. https://doi.org/10.11648/j.ijnrem.20180301.13

Pikovskaya, R.I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species’. Mikrobiologiya, 17, 362–370.

Pandey, A., Trivedi, P., Kumar, B. and Palni, L.M. (2006). Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a sub-Alpine location in the Indian Central Himalaya. Current Microbiology, 53(2), 102-7. https://doi.org/10.1007/s00284-006-4590-5

Steadman, K., Ellery, A., Chapman, R., Moore, A. and Turner, N. (2004). Maturation temperature and rainfall influence seed dormancy characteristics of annual ryegrass (Lolium rigidum). Australian Journal of Agricultural Research, 55(10), 1047-1057. https://doi.org/10.1071/AR04083

Stevenson, F.J. (2005). Cycles of Soil: Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients. John Wiley and Sons, Hoboken.

Subhashini, V. and Swamy, A.V.V.S. (2016). Efficiency of Echinochloa colona in the removal of heavy metals from contaminated soils. International Journal of Scientific Research, 5(3), 689. https://doi.org/10.36106/ijsr

Sánchez-Cruz, N.D., Meza-Contreras, J.C., Froylán, M.E., Macías-Rodríguez, M.E., Salcedo-Perez, E. and González-García, Y. (2020). Phosphate solubilization and indole-like compounds production by bacteria isolated from forest soil with plant growth promoting activity on pine seedlings. Geomicrobiology Journal, 37(10), 909-918. https://doi.org/10.1080/01490451.2020.1797945

Tangahu, B.V., Abdullah, S.R.S., Basri, H., Idris, M., Anuar, N. and Mukhlisin, M.A. (2011). Review on heavy metals (As, Pb, and Hg) uptake by plants through phytoremediation. International Journal of Chemical Engineering, 2011, 939161. https://doi.org/10.1155/2011/939161

Teng, Z., Shao, W., Zhang, K., Huo, Y. and Li, M. (2019). Characterization of phosphate solubilizing bacteria isolated from heavy metal contaminated soils and their potential for lead immobilization. Journal of Environmental Management, 231,189-197. https://doi.org/10.1016/j.jenvman.2018.10.012

Walpola, B.C. and Yoon, M.H. (2013). In Vitro solubilisation of inorganic phosphates by phosphate solubilizing microorganisms. African Journal of Microbiology Research, 7, 3534-3541.

Wan, W., Qin, Y., Wu, H., Zuo, W., He, H., Tan, J., Wang, Y. and He, D. (2020). Isolation and characterization of phosphorus solubilizing bacteria with multiple phosphorus sources utilizing capability and their potential for lead immobilization in soil. Frontier Microbiology, 11, 752. https://doi.org/10.3389/fmicb.2020.00752

Waterlot, C. (2018). Alternative approach to the standard, measurements and testing program used to establish phosphorus fractionation in soils. Analytic Climica Acta, 1003, 26–33. https://doi.org/10.1016/j.aca.2017.11.059

Xiao, C., Guo, S., Wang, Q. and Chi, R. (2021). Enhanced reduction of lead bioavailability in phosphate mining wasteland soil by a phosphate-solubilizing strain of Pseudomonas sp., LA, coupled with ryegrass (Lolium perenne L.) and sonchus (Sonchus oleraceus L.). Environmental Pollution, 274, 116572. https://doi.org/10.1016/j.envpol.2021.116572

Yahaghi, Z., Shirvani, M., Nourbakhsh, F., de la Peña, T.C., Pueyo, J.J. and Talebi, M. (2018). Isolation and characterization of Pb-solubilizing bacteria and their effects on Pb uptake by Brassica juncea: Implications for microbe-assisted phytoremediation. Journal of Microbiology and Biotechnology, 28(7), 1156-1167. https://doi.org/10.4014/jmb.1712.12038




DOI: http://dx.doi.org/10.14720/aas.2022.118.3.2454

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