Povečanje fitoremediacijske učinkovitosti vrste Echinochloa colona (L.) Link z bakterijami, ki sproščajo fosfor v tleh, onesnaženih s svincem

Quang Trung DO

Povzetek


Obetajoča rešitev za fitoremediacijo s kovinami onesnaženih tal je uporaba rastlin v kombinaciji z bakterijami, ki sproščajo fosfor (PSB). V raziskavi so bili preučevani izolati teh bakterij iz riževih polj in njihova sposobnost izboljšanja fitoremediacije svinca (Pb2+) s plevelno vrsto kostrebe (Echinochloa colona (L.) Link) kot tudi izboljšanje rasti te rastline v razmerah kovinskega stresa zaradi onesnaženja s svincem. Celokupno je bilo izoliranih 6 izolatov PSB (označenih kot TB01 do TB06), pri čemer je imel izolat TB04 največjo sposobnost sproščanja fosforja (z indeksom PSI = 7,13) iz kalcijevega fosfata (Ca3(PO4)2). Na sposobnost sproščanja fosforja pri sevu TB04 niso vplivale velike koncentracije Pb2+. Sev TB04 je bil identificiran kot vrsta bakterije Pseudomonas putida Trevisan, 1889 (številka akcesije FJ976601.1). Inokulacija kostrebe s sevom TB04 je značilno povečala njeno fitoremediacijsko učinkovitost za svinec v s svincem onesnaženih tleh, pri čemer je bila njena rast značilno pvečana. Rezultati nakazujejo, da bi sev TB04 lahko potencialno uporabili kot inokulant kostrebe kot nov način fitoremediacije s kovinami onesnaženih močvirnih tal.


Ključne besede


imobilizacija svinca; Pseudomonas putida; rodovitnost tal; fitoremediacija; s kovinami onesnažena tla

Celotno besedilo:

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Literatura


Aransiola, S.A., Ijah, U.J.J., Abioye, O.P. and Bala, J.D. (2019). Microbial-aided phytoremediation of heavy metals contaminated soil: a review. European Journal of Biological Research, 9(2), 104-125.

Adhikari, A., Lee, K.E., Khan, M.A., Kang, S.M., Adhikari, B., Imran, M., Jan, R., Kim, K.M. and Lee, I.J. (2020). Effect of silicate and phosphate solubilizing rhizobacterium Enterobacter ludwigii GAK2 on Oryza sativa L. under cadmium stress. Journal of Microbiology and Biotechnollogy, 30(1), 118-126. https://doi.org/10.4014/jmb.1906.06010

Fankem, H., Nwaga, D., Deubel, A., Dieng, L., Merbach, W. and Etoa, F.X. (2006). Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon. African Journal of Biotechnology, 5(24), 2450-2460.

Himani, S. and Reddy, M.S. (2011). Effect of inoculation with phosphate solubilizing fungus on growth and nutrient uptake of wheat and maize plants fertilized with rock phosphate in alkaline soils. European Journal of Soil Biology, 47(1), 30–34. https://doi.org/10.1016/j.ejsobi.2010.10.005

Jones, J.B. and Case, V.W. (1990). Sampling, handling, and analyzing plant tissue samples. In Westerman RL (ed.). Soil Testing and Plant Analysis. Soil Science Society of America, Inc., Madison, WI, pp. 389-447. https://doi.org/10.2136/sssabookser3.3ed.c15

Kumar, A. and Rai, L.C. (2015). Proteomic and biochemical basis for enhanced growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium. Microbiology Research, 170, 195-204. https://doi.org/10.1016/j.micres.2014.06.006

Katiyar, V. and Goel, R. (2003). Solubilization of inorganic phosphate and plant growth promotion by cold tolerant mutants of Pseudomonas fluorescens. Microbiology Research, 158(2), 163-168. https://doi.org/10.1078/0944-5013-00188

Liu, Z., Li, Y.C., Zhang, S., Fu, Y., Fan, X., Patel, J. S. and Zhang, M, (2015). Characterization of phosphate-solubilizing bacteria isolated from calcareous soils. Applied Soil Ecology, 96, 217–224. https://doi.org/10.1016/j.apsoil.2015.08.003

Lin, M., Jin, M., Xu, K., He, L. and Cheng, D. (2018). Phosphate-solubilizing bacteria improve the phytoremediation efficiency of Wedelia trilobata for Cu-contaminated soil. International Journal of Phytoremediation, 20(8), 813-822. https://doi.org/10.1080/15226514.2018.1438351

Marra, L.M., de Oliveira-Longatti, S.L., Cláudio R.F.S.S., Fábio L.O. and de Souza Moreira F.M. (2019). The amount of phosphate solubilization depends on the strain, C-source, organic acids and type of phosphate. Geomicrobiology Journal, 36(3), 232-242. https://doi.org/10.1080/01490451.2018.1542469

Nakhro, N. and Dkhar, M.S. (2010). Impact of organic and inorganic fertilizers on microbial population and biomass carbon in paddy field soil. Journal of Agronomy, 9(3), 102-110. https://doi.org/10.3923/ja.2010.102.110

Noble, A., Banakuna, F., Tanee, G. and Osuji, J. (2018). The effect of ripe plantain peels waste on the phytoextraction of Pb and Cd by Echinochloa colona (L.) Link. International Journal of Natural Resource Ecology and Management, 3(1), 19. https://doi.org/10.11648/j.ijnrem.20180301.13

Pikovskaya, R.I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species’. Mikrobiologiya, 17, 362–370.

Pandey, A., Trivedi, P., Kumar, B. and Palni, L.M. (2006). Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a sub-Alpine location in the Indian Central Himalaya. Current Microbiology, 53(2), 102-7. https://doi.org/10.1007/s00284-006-4590-5

Steadman, K., Ellery, A., Chapman, R., Moore, A. and Turner, N. (2004). Maturation temperature and rainfall influence seed dormancy characteristics of annual ryegrass (Lolium rigidum). Australian Journal of Agricultural Research, 55(10), 1047-1057. https://doi.org/10.1071/AR04083

Stevenson, F.J. (2005). Cycles of Soil: Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients. John Wiley and Sons, Hoboken.

Subhashini, V. and Swamy, A.V.V.S. (2016). Efficiency of Echinochloa colona in the removal of heavy metals from contaminated soils. International Journal of Scientific Research, 5(3), 689. https://doi.org/10.36106/ijsr

Sánchez-Cruz, N.D., Meza-Contreras, J.C., Froylán, M.E., Macías-Rodríguez, M.E., Salcedo-Perez, E. and González-García, Y. (2020). Phosphate solubilization and indole-like compounds production by bacteria isolated from forest soil with plant growth promoting activity on pine seedlings. Geomicrobiology Journal, 37(10), 909-918. https://doi.org/10.1080/01490451.2020.1797945

Tangahu, B.V., Abdullah, S.R.S., Basri, H., Idris, M., Anuar, N. and Mukhlisin, M.A. (2011). Review on heavy metals (As, Pb, and Hg) uptake by plants through phytoremediation. International Journal of Chemical Engineering, 2011, 939161. https://doi.org/10.1155/2011/939161

Teng, Z., Shao, W., Zhang, K., Huo, Y. and Li, M. (2019). Characterization of phosphate solubilizing bacteria isolated from heavy metal contaminated soils and their potential for lead immobilization. Journal of Environmental Management, 231,189-197. https://doi.org/10.1016/j.jenvman.2018.10.012

Walpola, B.C. and Yoon, M.H. (2013). In Vitro solubilisation of inorganic phosphates by phosphate solubilizing microorganisms. African Journal of Microbiology Research, 7, 3534-3541.

Wan, W., Qin, Y., Wu, H., Zuo, W., He, H., Tan, J., Wang, Y. and He, D. (2020). Isolation and characterization of phosphorus solubilizing bacteria with multiple phosphorus sources utilizing capability and their potential for lead immobilization in soil. Frontier Microbiology, 11, 752. https://doi.org/10.3389/fmicb.2020.00752

Waterlot, C. (2018). Alternative approach to the standard, measurements and testing program used to establish phosphorus fractionation in soils. Analytic Climica Acta, 1003, 26–33. https://doi.org/10.1016/j.aca.2017.11.059

Xiao, C., Guo, S., Wang, Q. and Chi, R. (2021). Enhanced reduction of lead bioavailability in phosphate mining wasteland soil by a phosphate-solubilizing strain of Pseudomonas sp., LA, coupled with ryegrass (Lolium perenne L.) and sonchus (Sonchus oleraceus L.). Environmental Pollution, 274, 116572. https://doi.org/10.1016/j.envpol.2021.116572

Yahaghi, Z., Shirvani, M., Nourbakhsh, F., de la Peña, T.C., Pueyo, J.J. and Talebi, M. (2018). Isolation and characterization of Pb-solubilizing bacteria and their effects on Pb uptake by Brassica juncea: Implications for microbe-assisted phytoremediation. Journal of Microbiology and Biotechnology, 28(7), 1156-1167. https://doi.org/10.4014/jmb.1712.12038




DOI: http://dx.doi.org/10.14720/aas.2022.118.3.2454

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