Effect of biofumigation by Calligonum polygonoides, dry olive leaves, and ash of olive leaves on chilli pepper growth and recovery of Rhizoctonia solani

Ahmed Abdel-Nabi AL-HAMMOURI, Salman AL-KOFAHI, Jwan IBBINI, Shereen ABUSMIER, Soum SANOGO


Rhizoctonia solani J.G. Kühn is a serious soilborne pathogen in chilli fields worldwide. This study examined the effect of biofumigation using arta (Calligonum polygonoides L.) and olive (Olea europaea L.) plant material on chilli growth and recovery of R. solani from chilli plants. The experiment was conducted under greenhouse conditions in potted soil amended with no plant material (control) or with plant material from Calligonum and Olea (olive leaves, or ash of olive leaves). Chilli was planted in the amended soils and inoculated with R. solani. Plant height, number of fruits , and frequency of recovery of R. solani from chilli were recorded. Soil amendment with Calligonum plant material resulted in the lowest frequency of recovery of R. solani. Moreover, Calligonum treatment increased dry mass and height of chilli plants compared to other treatments. Also, treatment with olive plant parts inhibited R. solani growth and enhanced growth of chilli compared to the control treatment. Thus, C. polygonoides and O. europaea are potental biofumigant plants to control R. solani.


chilli pepper; Capsicum annuum; ; biofumigation; Calligonum polygonoides; olive; Rhizoctonia solani; inoculation

Full Text:



Abeysinghe, S. (2009). Effect of combined use of Bacillus subtilis CA32 and Trichoderma harzianum RU01 on biological control or Rhizoctonia solani on Solanum melongena and Capsicum annuum. Plant Pathology Journal, 8, 9-16. https://doi.org/10.3923/ppj.2009.9.16

Abrahim, J., Afrah, E., M., Mudawi, M., E. (2014). Assessment of in-vitro anti-fungal potential of ethanolic extract of Calligonum comosum against two fungal postharvest pathogens of fruit and vegetables in Saudi Arabia. International Journal of Applied Biology and Pharmaceutical Technology (ijabpt), 5,90-94.

Aires, A., Carvalho, R., Barbosa, M. D. C., Rosa, E. (2009). Suppressing potato cyst nematode, Globodera rostochiensis, with extracts of Brassicacea plants. American Journal of Potato Research, 86, 327-333. https://doi.org/10.1007/s12230-009-9086-y

Alkhalifah, DHM, (2013). In-vitro antibacterial activity of ethanol extract of Calligonum comosum plant against four human pathogens in Saudi Arabia. International Journal of Plant, Animal and Environmental Sciences, 3, 170 -175.

Benavente-Garcia, O., Castillo, J., Lorente, J., Ortuno, A., Del Rio, J. (2000). Antioxidant activity of phenolics extracted from Olea europa L. leaves. Food Chemistry, 68, 457-462. https://doi.org/10.1016/S0308-8146(99)00221-6

Benitez, E; Melgar, R; Sainz, H; Gomez, M; Nogales, R. (2000). Enzyme activities in the rhizosphere of pepper (Capsicum annuum, L.) grown with olive cake mulches. Soil Biology & Biochemistry, 13(32), 1829-1835. https://doi.org/10.1016/S0038-0717(00)00156-5

Bubici, G., Amenduni, M., Colella, C., D’Amico, M., Cirulli, M. (2006). Efficacy of acibenzolar-S-methyl and two strobilurins, azoxystrobin and trifloxystrobin, for the control of corky root of tomato and Verticillium wilt of eggplant. Crop Protection, 25, 814-820. https://doi.org/10.1016/j.cropro.2005.06.008

Castillo, C. R., Sotomayor, L. S., Ortiz, C. O., Leonelli, G. C., Borie, F. B., Rubio, R. H. (2009). Effect of arbuscular mycorrhizal fungi on an ecological crop of chile peppers (Capsicum annuum L.). Chilean Journal of Agricultural Research, 69, 79-87. https://doi.org/10.4067/S0718-58392009000100010

Cimen, I., Basbag, S., Temiz, M., Sagir, A. (2004). The effect of paclobutrazol, growth retardant, on cotton growth and Verticillium wilt (Verticillium dahliae Kleb.). Plant Pathology Journal, 3, 35-39. https://doi.org/10.3923/ppj.2004.35.39

Fan, C. M., Isong, G. R., Qi, P., Ji, G. H., He, Y. Q. (2008). Potential biofumigation effects of Brassica oleracea var. caulorapa on growth of fungi. Journal of Phytopathology, 156, 321-325. https://doi.org/10.1111/j.1439-0434.2007.01343.x

Farid, A. B., Madiha, A., Mohamed, R. A. (2007). Evaluation of cytotoxic compounds from Calligonum comosum L. growing in Egypt. Zeitschrift für Naturforschung, 62, 656 – 660.

Ghazanfar, S. A. (1994). Handbook of Arabian Medicinal Plants. CRC Press, p.173.

Goldberg, N. P. (2001). Chilli pepper diseases. New Mexico State University, Colleg of Agricultural, Consumer and Environmental Sciences. Circular 549. Online publication. http://aces.nmsu.edu/pubs/_circulars/circ549.html. 6/27/2010.

Halis Y, Ed. (2007). Plant Encyclopedia in area Oued Souf: desert plants common in the Big East race, El Walid: 62-63.

Jarvis, W. R. (1993). Managing diseases in greenhouse crops. American Phytopathological Society Press. St Paul, MN.

Kamil M, Jayaraj AF, Ahmad F, Gunasekhar C, Samuel S, Habiballah M, Chan K, (2000). Pharmacognostic andphytochemical standardisation of Calligonum comosum. Journal of Pharmacy and Pharmacology, 52 (Suppl), 262 p.

Kaul, R. N. (1963). Need for afforestation in the arid zones of India. La-Yaaran, 13.

Kirkegaard, J. A., Sarwar, M., Wong, P. T. W., Mead, A., Howe, G., Newell, M. (2000). Field studies on the biofumigation of take-all by Brassica break crops. Australian Journal of Agricultural Research, 51, 445-456. https://doi.org/10.1071/AR99106

Laegdsmand, M., Gimsing, A. L., Strobel, B. W., Sǿrensen, J. C., Jacobsen, O. H., Christian, H., Hansen, B. (2007). Leaching of isothiocyanates through intact soil following simulated biofumigation. Plant Soil, 291, 81-92. https://doi.org/10.1007/s11104-006-9176-2

Larkin, R. P., Griffin, T. S. (2007). Control of soilborne potato diseases using Brassica green manures. Crop Protection, 26, 1067-1077. https://doi.org/10.1016/j.cropro.2006.10.004

Lazarovits, G., Conn, K. L., Abbasi, P. A., Soltani, N., Kelly, W., McMillan, E., Peters, R. D., Drake, K. A. (2008). Reduction of potato tuber diseases with organic soil amendments in two Prince Edward Island fields. Canadian Journal of Plant Patholology, 30, 37-45. https://doi.org/10.1080/07060660809507494

Matthiessen, J. N., Kirkegaard, J. A. (2006). Biofumigation and enhanced biodegradation: opportunity and challenge in soilborne pest and disease management. Critical Reviews in Plant Sciences, 25, 235-265. https://doi.org/10.1080/07352680600611543

Nogales, R., Melgar, Benetiz, E. (2006). Potential use of olive-waste ash from congeneration plants as a soil amendment. Journal of Environmental Science and Health Part B, 41, 1405-1415. https://doi.org/10.1080/03601230600964282

Pinelli, P., Galardi, C., Mulinacci, N., Vincieri, F., Tattini, ., Romani, A .(2000). Quali-quantitative analysis and antioxidant activity of different polyphenolic extracts from Olea europea L. leaves. Journal of Commodity Science, 39(2), 71-83.

Porras, M., Barrau, C., Romero, E., Zurera, C., Romero, F. (2009). Effect of biofumigation with Brassica carinata and soil solarization on Phytophthora spp. and strawberry. Yield. Proc. VIth Internat. Strawberry Symposium; Huelva, Spain, March 3-7, 2008. Acta Horticulturae, 842, 969-972. https://doi.org/10.17660/ActaHortic.2009.842.215

Powelson, M. L., Rowe, R. C. (1993). Biology and management of early dying of potatoes. Annual Review of Phytopathology, 31, 111-126. https://doi.org/10.1146/annurev.py.31.090193.000551

Romero, E.; Benitez, E.; Nogales, R. (2005). Suitability of wastes from olive-oil industry for initial reclamation of a Pb/Zn mine tailing. Water, Air, & Soil Pollution, 165, 153–165. https://doi.org/10.1007/s11270-005-4638-3

Sanogo, S. (2003). Chile pepper and the threat of wilt diseases. Plant Health Progress. https://doi.org/10.1094/PHP-2003-0430-01-RV

Sanogo, S., Carpenter, J. (2006). Incidence of Phytophthora blight and Verticillium wilt within chilli pepper fields in New Mexico. Plant Diseases, 90, 291-296. https://doi.org/10.1094/PD-90-0291

Scholberg, J. M., Zotarelli, L., Tubbs, R. S., Dukes, M. D., Muñoz-Carpena, R. (2009). Nitrogen uptake efficiency and growth of bell pepper in relation to time of exposure to fertilizer solution. Communications in Soil Science and Plant Analysis, 40, 2111-2131. https://doi.org/10.1080/00103620902960625

Siddiqui, I. A., Shaukat, S. S. (2005). Phenylacetic acid-producing Rhizoctonia solani represses the biosynthesis of nematicidal compounds in vitro and influences biocontrol of Meloidogyne incognita in tomato by Pseudomonas fluorescens strain CHA0 and its GM derivatives. Journal of Applied Microbiology, 98, 43-55. https://doi.org/10.1111/j.1365-2672.2004.02457.x

Skaggs, R., Decker, M., VanLeeuwen, D. (2000). A survey of southern New Mexico chilli producers: Production practices and problems. NM Agricultural Experiment Station Technology Bulletin., 782.

Snapp, S. S., Date, K. U., Kirk, W., O'Neil, K., Kermen, A., Bird, G. (2007). Root, shoot tissues of Brassica juncea and cereal secale promote potato health. Plant Soil, 294, 55-72. https://doi.org/10.1007/s11104-007-9228-2

Solomon, T. E., Lodolini, E. M., Neri, D. (2015). Effects of olive shoot residues on shoot and root growth of potted olive plantlets. Scientia Horticulturae, 182, 31-40. https://doi.org/10.1016/j.scienta.2014.11.008

Sultana, V., Ara, J., Ehteshamul-Haque, S. (2008). Suppression of root rotting fungi and root knot nematode of chile by seaweed and Pseudomonas aeruginosa. Journal of Phytopathology, 156, 390-359. https://doi.org/10.1111/j.1439-0434.2007.01369.x

Tariq, S., Khan, R., Sultana, V., Ara, J., Ehteshamul-Haque, S. (2009). Utilization of endo-root fluorescent Pseudomonas of chilli for the management of root diseases of chilli. Pakistan Journal of Botany, 41, 3191-3198.

Vivioli, F., Bellomo, G. Galli, C. (1998). Free radical-scavenging properties of olive oil polyphenols. Biochemical and Biophysical Research Communications, 247, 60-64. https://doi.org/10.1006/bbrc.1998.8735

Woodhall, J. W., Lees, A. K., Edwards, S. G., Jenkinson, P. (2007). Characterization of Rhizoctonia solani from potato in Great Britain. Plant Pathology, 56, 286-295. https://doi.org/10.1111/j.1365-3059.2006.01545.x

Zaki, D., Abd-El-Aziz, M., El-Gengeihy, S., Morsi, N. (1984). Antimicrobial potentialities of some Egyptian desert plants. Herba Hungarica, 23, 73 – 84.

DOI: http://dx.doi.org/10.14720/aas.2018.111.1.05


  • There are currently no refbacks.

Copyright (c) 2018 Ahmed Abdel-Nabi Al-Hammouri, Salman Al-Kofahi, Jwan Ibbini, Shereen Abusmier, Soum Sanogo

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.


eISSN 1854-1941