Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)



Ribonucleic acid (RNA) quality and integrity are crucial for many studies in plant molecular biology. High-quality RNA extraction from plants with high levels of compounds such as polysaccharides, polyphenols, and other secondary metabolites are problematic. RNA extraction from Lemon balm tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds or can be done by expensive protocols. This study shows improvement of a CTAB-based protocol which allows rapid and easy isolation of high-quality RNA from Lemon balm plant. The RNA obtained is suitable for cDNA synthesis and RT-PCR experiments.


Melissa officinalis; CTAB; RNA; RT-PCR

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Chang, S., Puryear J., Cairney J. (1993). A simple and efficient method for isolating RNA from pine trees. Plant molecular biology reporter, 11(2), 113-116.

Döring, A.S., Pellegrini, E., Della Batola, M., Nali, C., Lorenzini, G., Petersen, M. (2014). How do background ozone concentrations affect the biosynthesis of rosmarinic acid in Melissa officinalis?. Journal of plant physiology, 171(5), 35-41.

Gambino, G., Perrone, I., Gribaudo, I. (2008). A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants. Phytochemical Analysis, 19(6), 520-525.

Guerriero, G., Mangeot-Peter, L., Hausman, J.F., Legay, S. (2016). Extraction of high quality RNA from Cannabis sativa bast fibres: A vademecum for molecular biologists. Fibers, 4(3), 23.

Hou, P., Xie, Z., Zhang, L., Song, Z., Mi, J., He, Y., Li, Y. (2011). Comparison of three different methods for total RNA extraction from Fritillaria unibracteata, a rare Chinese medicinal plant. Journal of Medicinal Plants Research, 5(13), 2835-2839.

Hunter, D.A., & Reid, M.S. (1999). A simple and rapid method for isolating high quality RNA from flower petals. In VII International Symposium on Postharvest Physiology of Ornamental Plants, 543, 147-152.

Iandolino, A.B., Da Silva, F.G., Lim, H., Choi, H., Williams, L.E., Cook, D.R. (2004). High-quality RNA, cDNA, and derived EST libraries from grapevine (Vitis vinifera L.). Plant Molecular Biology Reporter, 22(3), 269-278.

Jafarpour, M., & Fard, A.N. (2016). The effects of aqueous extract of Melissa officinalis on some blood parameters and liver of Oncorhynchus mykiss. Aquaculture, Aquarium, Conservation & Legislation-International Journal of the Bioflux Society (AACL Bioflux), 9(3).

Jalal, Z., El Atki, Y., Lyoussi, B., Abdellaoui, A. (2015). Phytochemistry of the essential oil of Melissa officinalis L. growing wild in Morocco: preventive approach against nosocomial infections. Asian Pacific Journal of Tropical Biomedicine, 5(6), 458-461.

Jordon-Thaden, I. E., Chanderbali, A. S., Gitzendanner, M. A., Soltis, D. E. (2015). Modified CTAB and TRIzol protocols improve RNA extraction from chemically complex Embryophyta. Applications in plant sciences, 3(5), 1400105.

Jun, H. J., Lee, J. H., Jia, Y., Hoang, M. H., Byun, H., Kim, K. H., Lee, S. J. (2012). Melissa officinalis essential oil reduces plasma triglycerides in human apolipoprotein E2 transgenic mice by inhibiting sterol regulatory element-binding protein-1c–dependent fatty acid synthesis. The Journal of nutrition, 142(3), 432-440.

Kim, J. H., Hossain, A. M., Kim, N. H., Lee, D. H., Lee, H. J. (2011). Identification and functional characterization of the GALACTINOL SYNTHASE (MoGolS1) gene in Melissa officinalis plants. Journal of Applied Biological Chemistry, 54(4), 244-251.

Liao, C. M., Li, J., Liu, X. H., Zhang, Y. S. (2014). An effective method for extracting total RNA from Dioscorea opposita Thunb. Genetics and Molecular Research, 13, 462-468.

Martínez-Fuentes, A., Mesejo, C., Agustí, M., Reig, C. (2015). Toward a more efficient isolation of total RNA from loquat (Eriobotrya japonica Lindl.) tissues. Fruits, 70(1), 47-51.

Meisel, L., Fonseca, B., González, S., Baeza-Yates, R., Cambiazo, V., Campos, R., Gonzalez, M., Orellana, A., Retamales, J., Silva, H. (2005). A rapid and efficient method for purifying high quality total RNA from peaches (Prunus persica) for functional genomics analyses. Biological research, 38(1), 83-88.

Nasiri-Bezenjani, M. A., Riahi-Madvar, A., Baghizadeh, A., Ahmadi, A. R. (2014). Rosmarinic acid production and expression of tyrosine aminotransferase gene in Melissa officinalis seedlings in response to yeast extract. Journal of Agricultural Science and Technology, 16(4), 921-930.

Ouyang, K., Li, J., Huang, H., Que, Q., Li, P., Chen, X. (2014). A simple method for RNA isolation from various tissues of the tree Neolamarckia cadamba. Biotechnology & Biotechnological Equipment, 28(6), 1008-1013.

Rubio-Piña, J. A., & Zapata-Pérez, O. (2011). Isolation of total RNA from tissues rich in polyphenols and polysaccharides of mangrove plants. Electronic journal of Biotechnology, 14(5), 11-11.

Sabzevari, A. G., & Hosseini, R. (2014). A quick, efficient, and cost-effective method for isolating high-quality total rna from tomato fruits, suitable for molecular biology studies. Preparative Biochemistry and Biotechnology, 44(4), 418-431.

Saraydin, S. U., Tuncer, E., Tepe, B., Karadayi, S., Ozer, H., Sen, M., Karadayi, K., Inan, D., Elagoz, S., Polat, Z., Duman, M. (2012). Antitumoral effects of Melissa officinalis on breast cancer in vitro and in vivo. Asian Pacific journal of cancer prevention, 13(6), 2765-2770.

Shu, C., Sun, S., Chen, J., Chen, J., Zhou, E. (2014). Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani. Electronic Journal of Biotechnology, 17(1), 50-54.

Wahby, M. (2016). Effect of drying period and harvesting times on herb, essential oil content and its constituent’s from different parts of Melissa officinalis. Journal of Chemical and Pharmaceutical Research, 8(5), 919-933.



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