Modified CTAB protocol for RNA extraction from Lemon balm (Melissa officinalis L.)

Fatemeh RAHMANI, Leila AMRAEE

Abstract


Ribonucleic acid (RNA) quality and integrity are crucial for many studies in plant molecular biology. High-quality RNA extraction from plants with high levels of compounds such as polysaccharides, polyphenols, and other secondary metabolites are problematic. RNA extraction from Lemon balm tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds or can be done by expensive protocols. This study shows improvement of a CTAB-based protocol which allows rapid and easy isolation of high-quality RNA from Lemon balm plant. The RNA obtained is suitable for cDNA synthesis and RT-PCR experiments.


Keywords


Melissa officinalis; CTAB; RNA; RT-PCR

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References


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DOI: http://dx.doi.org/10.14720/aas.2020.115.1.692

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