Določanje in opis endofitskih bakterij, ki povzročajo oljkovega raka na mladih oljkah

Maryam KHEZRI, Abolghasem GHASEMI, Akbar AHANGARAN

Povzetek


Oljkov rak je pomembna bolezen v vseh deželah, kjer gojijo oljke. Spomladi leta 2015 so bili v severnem Iranu na deblih in vejah štiriletnih oljk opaženi tumorji. Iz njih so bile izolirane gram negativne bakterije, ki so bile sposobne tvoriti fluorescentni pigment. Ocenjene so bile tudi druge fenotipske lastnosti izolatov. Test patogenosti različnih izolatov je bil opravljen na oljčnih vejah. Prva bolezenska znamenja so se pojavila po dveh tednih. Zaporedja 16S rRNK in genov beta podenote RNK polimeraze iz patogenih izolatov so bila popolnoma podobna tistim iz bakterije Pseudomonas savastanoi pv. savastanoi  (Smith 1908) Young et al. 1978, iz GenBank. Na podlagi rezultatov fenotipskih analiz, testov patogenosti in filogenetskih podatkov so bili izolati določeni kot vrsta P. savastanoi pv. savastanoi. Gostitelji izoliranih bakterij so bile samo oljke. Na nobeni od inokuliranih drugih vrst, kot so zimski jasmin (Jasminum nudiflorum Lindl.), navadni oleander (Nerium oleander L.), japonska kalina (Ligustrum japonicum Thunb.) in veliki jesen (Fraxinus excelsior L.), se niso razvila bolezenska znamenja. Med šestimi preučevanimi sortami oljk ni bilo razlik v odpornosti proti bolezeni. V okolici preučevanega oljčnika ni bilo v razdalji več kot kilometer nobene oljke, niti oljčnika. Povzročitelj bolezni je v Iranu na seznamu tujerodnih karantenskih škodljivcev in bolezni, zato so bili sprejeti ustrezni karantenski in fitosanitarni ukrepi za izkoreninjenje bolezni.

Ključne besede


Pseudomonas savastanoi pv. savastanoi; fenotipska identifikacija; 16S rRNA, rpoB; sortna odpornost

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2019.113.1.10

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