Expression of IRT1 gene in barley seedlings under zinc deficiency at optimal and low temperatures

Natalia KAZNINA, Natalia REPKINA, Yulia BATOVA, Alexandr TITOV

Abstract


The deficiency or excess of zinc (Zn) cause negative effect on plant metabolism and development. Therefore, plants have established a tightly controlled system, including protein transporters to balance the uptake and utilization of metal ions. In this study, the relative expression of HvIRT1 gene, encoding the transmembrane protein IRT1 was analyzed in shoots and roots of barley (Hordeum vulgare ‘Nur’) under zinc deficiency at optimal (22 °C) or low (4 °C) temperatures. The Zn deficiency (0 μmol) caused an increase in HvIRT1 gene expression under both optimal temperature condition and cold. Although, the difference in mRNA content of HvIRT1 gene in roots of barley under optimal and low temperature was not observe. However, the HvIRT1 expression in leaves was higher at optimal temperature compare with cold condition. Moreover, long-term (7 days) of low temperature influence along with zinc deficiency leads to a significant decrease in the amount of HvIRT1transcripts in leaves, that corresponds to a decrease of photosynthesis rate and biomass accumulation. Overall, these findings suggest that HvIRT1 gene play an important role in plant’s response to zinc deficiency under optimal temperatures condition as well as at cold.


Keywords


IRT1; Hordeum vulgare; zinc deficiency; low temperatures

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References


Brumbarova, T, Bauer, P, Ivanov, R (2015). Molecular mechanisms governing Arabidopsis iron uptake. Trends in Plant Science, 20(2), 124–133. https://doi.org/10.1016/j.tplants.2014.11.004

Giehl, R. F. H., Meda, A.R., von Wirén, N. (2009). Moving up, down, and everywhere: signaling of micronutrients in plants. Cur-rent Opinion in Plant Biology, 12, 320–327. https://doi.org/10.1016/j.pbi.2009.04.006

Grotz,N., Fox, T., Connolly, E., Park, W., Guerinot, M.L., Eide, D. (1998). Identification of a family of zinc transporter genes from Arabidopsis that respond to zinc deficiency. Proceedings of the National. Academy of Sciences USA, 95, 7220–7224. https://doi.org/10.1073/pnas.95.12.7220

Guerinot,M.L.(2000). The ZIP family of metal transporters. Biochimica et Biophysica Acta, 1465, 190–198. https://doi.org/10.1016/S0005-2736 (00)00138-3

Hacisalihoglu,G., Hart, J.J., Kochian, L.V. (2001). High- and low- affinity zinc transport systems and their possible role in zinc efficiency in bread wheat. Plant Physiology, 125, 456–463. https://doi.org/10.1104/pp.125.1.456

Hajiboland, R., Beiramzadeh, N. (2008). Growth, gas exchange and function of antioxidant defense system in two contrasting rice genotypes under Zn and Fe deficiency and hypoxia. Acta Bioogica. Szegediensis, 52(2), 283–294. http://www.sci.u-szeged.hu/ABS

Ishimaru, Y., Suzuki, M., Tsukamoto, T., et al. (2006). Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+.The Plant Journal, 45, 335–346. https://doi.org/10.1111/j.1365-313X.2005.02624.x

Kabir, A. H., Hossain, M.M., Khatun, M.A., Sarkar, M.R., Haider, S.A. (2017). Biochemical and molecular mechanisms associated with Zn deficiency tolerance and signaling in rice (Oryza sativa L.). Journal of Plant Interactions, 12(1), 447–456. https://doi.org/10.1080/17429145.2017.1392626

Kaznina, N.M., Titov, A.F., Repkina, N.S., Batova, Yu.V. (2019). Effect of zinc excess and low temperature on the IRT1 gene expression in the roots and leaves of barley. Doklady Biochemistry and Biophysics, 48, 264–268. https://doi.org/10.1134/S1607672919040057

Lee, S., A., G. (2009). Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice. Plant, Cell and Environ-ment, 32, 408–416. https://doi.org/10.1111/j.1365-3040.2009.01935.x

Li, Y., Zhang, Y., Shi, D., Kiu, X., Qin, J, Ge, Q. … Xu, J. (2013). Spatial-temporal analysis of zinc homeostasis reveals the response mechanisms to acute zinc deficiency in Sorghum bicolor. New Phytologist, 200, 1102–1115. https://doi.org/10.1111/nph.12434

Liu, H., Gan, W., Renge, Z., Zhao, P. (2016). Effects of zinc fertilizer rate and application method on photosynthetic characteris-tics and grain yield of summer maize. Journal of Soil Science and Plant Nutrition, 16(2), 550–662. https://doi.org/10.4067/S0718-95162016005000045

Livak, K.J., Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔct method. Methods, 25, 402–408. https://doi.org/10.1006/meth.2001.1262

Palmer, G.M., Guerinot, M.L. (2009). Facing the challenges of Cu, Fe and Zn homeostasis in plants. Nature Chemical Biology, 5, 333–340. https://doi.org/10.1038/nchembio.166

Pedas, P., Ytting, C. K., Fuglsang, A. T., Jahn, T. P., Schioerring, J. K., Hasted, S. (2008). Manganese efficiency in barley: identifi-cation and characterization of the metal ion transporter HvIRT11[OA]. Plant Physiology, 148, 455–466. https://doi.org/10.1104/pp.108.118851

Romera, F. J., García, M. J., Alcántara, E., Pérez-Vicente, R. (2011). Latest findings about the interplay or auxin, ethylene and nitric oxide in the regulation of Fe deficiency responses by strategy I plants. Plant Signaling and Behavior, 6, 167–170. https://doi.org/10.4161/psb.6.1.14111

Shin, L.-J., Lo, J.-C., Chen, G. H., Callis, J., Fu, H., Yeh, K-C. (2013). IRT1 degradation factor1, a ring E3 ubiquitin ligase, regulates the degradation of iron-regulated transporter1 in Arabidopsis. The Plant Cell, 25, 3039–3051. https://doi.org/10.1105/tpc.113.115212

Suzuki, M., Bashir, K., Inoue, H., Takahashi, M., Nakanishi, H., Nishizawa, N.K. (2012). Accumulation of starch in Zn-deficient rice. Rice, 59, 1–8. https://doi.org/10.1186/1939-8433-5-9

Yamunarani, R., Ramegowda, V., Pavithra, J., Geetha, G., Rajashekar-Reddy, H., Udayakumar, M., Shankar, A. G. (2013). Expression of a rice Zn transporter, OsZIP1, increases Zn concentration in tobacco and finger millet transgenic plants. Plant Biotechnology Reports, 7, 309–319. https://doi.org/10.1007/s11816-012-0264-x




DOI: http://dx.doi.org/10.14720/aas.2021.117.4.1998

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