Genetske raziskave soje [Glycine max (L.) Merr.] v povezavi s stresnimi dejavniki pri shranjevanju semena

Gbemisola Oluseun OKUNLOLA, Emmanuel IDEHEN, Johnson Adedayo ADETUMBI, Christopher ALAKE, Solomon Tayo AKINYOSOYE, Oluwafemi Daniel AMUSA

Povzetek


Genotipi soje (Glycine max (L.) Merill) se odzivajo različno na stresne dejavnike med shranjevanjem semena. Namen raziskave je bil oceniti genetski potencial 15 sort semen soje na stres med shranjevanjem pri pospešenih tehnikah staranja. Genotipi so bili razvrščeni na osnovi njihove tolerance na stres med staranjem na osnovi enostavnih ponavljajočih se zaporedij (SSR) in agronomskih lastnosti. Toleranca 15 sort semen soje na stres med shranjevanjem je bila ocenjenaz izpostavitvijo pospešenemu staranju za 0, 6, 12, in 24 ur pri 42 °C in 100 % relativni vlažnosti (RH). Nekatere od sort so bile shranjene tudi za 6 mesecev v razmerah 65 ± 5 % relativne vlažnosti in  pri 25 ± 2 °C. Ti genotipi so bili ovrednoteni z markerji na osnovi19 enostavnih ponavljajočih se zaporedij (SSR). Sorte so se združevale na osnovi njihove tolerance na stress med shranjevanjem. Analiza glavnih komponent je pokazala (PCA), da sta bila indeks hitrosti kalitve (GRI) in kalitveni indeks (GI) najpomembnejša pokazatelja značilne variabilnosti v lastnostih, ki označujejo vigor sejank. TGX1835-10E in TGX1448-2E sta bili prepoznani kot sorti z dobro sposobnostjo shranjevanja in sta priporočeni v žlahtniteljskih programih soje za izboljševanje shranjevanja semena. Šest SSR markerjev (Satt 565, Satt 175, Satt 281, Satt 600, Satt 160 and Satt 281)je bilo prepoznanih kot primernih za ugotavljanje alelov odgovornih za tolerance na stres med shranjevanjem.


Ključne besede


soja; pospešeno staranje semen; analiza glavnih komponent (PCA); dendrogram; SSR markerji; toleranca

Celotno besedilo:

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DOI: http://dx.doi.org/10.14720/aas.2020.116.1.1181

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