Velika odpornost navadnega prosa (Panicum miliaceum L.) na strupenost fenantrena temelji na rastnem odzivu in antioksidacijskem sistemu

Sarieh TARIGHOLIZADEH, Rouhollah MOTAFAKKERAZAD, Seyed Yahya SALEHI-LISAR, Elham MOHAJEL KAZEMI

Povzetek


Policiklični aromatski ogljikovodiki so skupina organskih onesnažil, ki vplivajo na različne vidike fiziologije rastlin. Fiziološki odzivi, povezani z vplivom fenantrena (PHE; 500, 1000, 1500, 2000 ppm) so bili analizirani na navadnem prosu (Panicum miliaceum L.). Kalitev semen je bila zapoznela pri vseh obravnavanjih, koncentracija fenantrena 2000 ppm je značilno zmanjšala hitrost kalitve (28 %) v primerjavi s kontrolo. Rezultati so pokazali, da se je negativni učinek fenantrena na rastne parametre in fotosintezena barvila v koncentracijah 1500 in 2000 ppm pokazal šele po 30 dnevih gojenja. Rastline, ki so bile izpostavljene koncentracijam 500 in 1000 ppm PHE so pokazale povečanje v rastnih parametrih, brez vsakršnih znakov zastrupitve, kar kaže na veliko tolerance teh rastlin na fenantren. V obravnavanih rastlinah se je povečala aktivnost antioksidacijskih encimov. Pri obravnavanjih z večjimi koncentracijami se je povečala tudi koncentracija H2O2, kljub zmanjšanju vsebnosti malondialdehida. Dodatno, PHE ni imel nobenega učinka na vsebnost fenolov v koreninah in flavonoidov v poganjkih, kot tudi ne na vsebnost beljakovin v koreninah in poganjkih. Zaključimo lahko, da fenantren ni bil zelo strupen za navadno proso, verjetno zaradi večje aktivnosti antioksidacijskega sistema, ki je preprečil tvorbo ROS, celo pri rastlinah obravnavanimi z večjimi koncentracijami PHE.

Ključne besede


policiklični aromatski ogljikovodiki; Panicum miliaceum; fenantren; fiziološki odziv; strupenost

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Literatura


Afegbua, S. L., & Batty, L. C. (2018). Effect of single and mixed polycyclic aromatic hydrocarbon contamination on plant biomass yield and PAH dissipation during phytoremediation. Environmental Science and Pollution Research, 25(19), 18596-18603. https://doi.org/10.1007/s11356-018-1987-1

Ahammed, G. J., Yuan, H. L., Ogweno, J. O., Zhou, Y. H., Xia, X. J., Mao, W. H., ... & Yu, J. Q. (2012). Brassinosteroid alleviates phenanthrene and pyrene phytotoxicity by increasing detoxification activity and photosynthesis in tomato. Chemosphere, 86(5), 546-555. https://doi.org/10.1016/j.chemosphere.2011.10.038

Alkio, M., Tabuchi, T. M., Wang, X., & Colon-Carmona, A. (2005). Stress responses to polycyclic aromatic hydrocarbons in Arabidopsis include growth inhibition and hypersensitive response-like symptoms. Journal of Experimental Botany, 56(421), 2983-2994. https://doi.org/10.1093/jxb/eri295

Alscher, R. G., Donahue, J. L., & Cramer, C. L. (1997). Reactive oxygen species and antioxidants: relationships in green cells. Physiologia Plantarum, 100(2), 224-233. https://doi.org/10.1111/j.1399-3054.1997.tb04778.x

Boominathan, R., & Doran, P. M. (2002). Ni‐induced oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii. New phytologist, 156(2), 205-215. https://doi.org/10.1046/j.1469-8137.2002.00506.x

Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Chance, B., & Maehly, A. C. (1955). [136] Assay of catalases and peroxidases. https://doi.org/10.1002/9780470110171.ch14

Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10(3). https://doi.org/10.38212/2224-6614.2748

Desalme, D., Binet, P., Epron, D., Bernard, N., Gilbert, D., Toussaint, M. L., ... & Chiapusio, G. (2011). Atmospheric phenanthrene pollution modulates carbon allocation in red clover (Trifolium pratense L.). Environmental pollution, 159(10), 2759-2765. https://doi.org/10.1016/j.envpol.2011.05.015

Di Giulio, R. T. (1991). Indices of oxidative stress as biomarkers for environmental contamination. In Aquatic Toxicology and Risk Assessment: Fourteenth Volume. ASTM International. https://doi.org/10.1520/STP23561S

Dong, Y.C., Zheng, D. S. (2006). Crops and their wild relatives in China.

Dupuy, J., Leglize, P., Vincent, Q., Zelko, I., Mustin, C., Ouvrard, S., & Sterckeman, T. (2016). Effect and localization of phenanthrene in maize roots. Chemosphere, 149, 130-136. https://doi.org/10.1016/j.chemosphere.2016.01.102

Dupuy, J., Ouvrard, S., Leglize, P., & Sterckeman, T. (2015). Morphological and physiological responses of maize (Zea mays) exposed to sand contaminated by phenanthrene. Chemosphere, 124, 110-115. https://doi.org/10.1016/j.chemosphere.2014.11.051

Habiyaremye, C., Barth, V., Highet, K., Coffey, T., & Murphy, K. M. (2017). Phenotypic responses of twenty diverse proso millet (Panicum miliaceum L.) accessions to irrigation. Sustainability, 9(3), 389. https://doi.org/10.3390/su9030389

Hamdi, H., Benzarti, S., Manusadžianas, L., Aoyama, I., & Jedidi, N. (2007). Bioaugmentation and biostimulation effects on PAH dissipation and soil ecotoxicity under controlled conditions. Soil Biology and Biochemistry, 39(8), 1926-1935. https://doi.org/10.1016/j.soilbio.2007.02.008

Harinasut, P., Poonsopa, D., Roengmongkol, K., & Charoensataporn, R. (2003). Salinity effects on antioxidant enzymes in mulberry cultivar. Science Asia, 29(2), 109-113. https://doi.org/10.2306/scienceasia1513-1874.2003.29.109

Hartmut, K. L. (1987). Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. In R. D. Lester Packer (Ed.). Methods in enzymology. New York, NY, Academic Press, pp 350–382. http://dx.doi.org/10.1016/0076-6879(87)48036-1

Henner, P., Schiavon, M., Druelle, V., & Lichtfouse, E. (1999). Phytotoxicity of ancient gaswork soils. Effect of polycyclic aromatic hydrocarbons (PAHs) on plant germination. Organic geochemistry, 30(8), 963-969. https://doi.org/10.1016/S0146-6380(99)00080-7

Houshani, M., Salehi-Lisar, S. Y., Movafeghi, A., & Motafakkerazad, R. (2019). Growth and antioxidant system responses of maize (Zea mays L.) seedling to different concentration of pyrene in a controlled environment. Acta agriculturae Slovenica, 113(1), 29-39. http://dx.doi.org/10.14720/aas.2019.113.1.03

Hu, X., Wang, J., Lu, P., & Zhang, H. (2009). Assessment of genetic diversity in broomcorn millet (Panicum miliaceum L.) using SSR markers. Journal of Genetics and Genomics, 36(8), 491-500. https://doi.org/10.1016/S1673-8527(08)60139-3

International Agency for Research on Cancer. (1983). Polynuclear aromatic compounds, part 1, chemical, environmental, and experimental data. IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Man, IARC Scientific Publications, 32, 33-451.

Krzebietke, S. J., Wierzbowska, J., Żarczyński, P. J., Sienkiewicz, S., Bosiacki, M., Markuszewski, B., ... & Mackiewicz-Walec, E. (2018). Content of PAHs in soil of a hazel orchard depending on the method of weed control. Environmental monitoring and assessment, 190(7), 422. https://doi.org/10.1007/s10661-018-6812-2

Kummerová, M., & Kmentová, E. (2004). Photoinduced toxicity of fluoranthene on germination and early development of plant seedling. Chemosphere, 56(4), 387-393. https://doi.org/10.1016/j.chemosphere.2004.01.007

Kummerová, M., Zezulka, Š., Babula, P., & Váňová, L. (2013). Root response in Pisum sativum and Zea mays under fluoranthene stress: morphological and anatomical traits. Chemosphere, 90(2), 665-673. https://doi.org/10.1016/j.chemosphere.2012.09.047

Kummerová, M., Zezulka, Š., Váňová, L., & Fišerová, H. (2012). Effect of organic pollutant treatment on the growth of pea and maize seedlings. Open Life Sciences, 7(1), 159-166. https://doi.org/10.2478/s11535-011-0081-1

Li, J. H., Gao, Y., Wu, S. C., Cheung, K. C., Wang, X. R., & Wong, M. H. (2008). Physiological and biochemical responses of rice (Oryza sativa L.) to phenanthrene and pyrene. International journal of phytoremediation, 10(2), 106-118. https://doi.org/10.1080/15226510801913587

Li, Q., Lu, Y., Shi, Y., Wang, T., Ni, K., Xu, L., ... & Giesy, J. P. (2013). Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings. Journal of Environmental Sciences, 25(9), 1936-1946. https://doi.org/10.1016/S1001-0742(12)60264-2

Liu, H., Weisman, D., Ye, Y. B., Cui, B., Huang, Y. H., Colón-Carmona, A., & Wang, Z. H. (2009). An oxidative stress response to polycyclic aromatic hydrocarbon exposure is rapid and complex in Arabidopsis thaliana. Plant Science, 176(3), 375-382. https://doi.org/10.1016/j.plantsci.2008.12.002

Liu, M., Qiao, Z., Zhang, S., Wang, Y., & Lu, P. (2015). Response of broomcorn millet (Panicum miliaceum L.) genotypes from semiarid regions of China to salt stress. The Crop Journal, 3(1), 57-66. https://doi.org/10.1016/j.cj.2014.08.006

Mann, T. (1984). A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye-binding. Annals of Biochemistry, 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3

Miliauskas, G., Venskutonis, P. R., & Van Beek, T. A. (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food chemistry, 85(2), 231-237. https://doi.org/10.1016/j.foodchem.2003.05.007

Mita, S., Murano, N., Akaike, M., & Nakamura, K. (1997). Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for β‐amylase and on the accumulation of anthocyanin that are inducible by sugars. The Plant Journal, 11(4), 841-851. https://doi.org/10.1046/j.1365-313X.1997.11040841.x

Mojiri, A., Ziyang, L., Tajuddin, R. M., Farraji, H., & Alifar, N. (2016). Co-treatment of landfill leachate and municipal wastewater using the ZELIAC/zeolite constructed wetland system. Journal of environmental management, 166, 124-130. https://doi.org/10.1016/j.jenvman.2015.10.020

Na, X., Cao, X., Ma, C., Ma, S., Xu, P., Liu, S., ... & Qiao, Z. (2019). Plant stage, not drought stress, determines the effect of cultivars on bacterial community diversity in the rhizosphere of broomcorn millet (Panicum miliaceum L.). Frontiers in microbiology, 10, 828. https://doi.org/10.3389/fmicb.2019.00828

Obinger, C., Maj, M., Nicholls, P., & Loewen, P. (1997). Activity, Peroxide Compound Formation, and Heme d Synthesis in Escherichia coli HPII Catalase. Archives of Biochemistry and Biophysics, 342(1), 58-67. https://doi.org/10.1006/abbi.1997.9988

Oguntimehin, I., Eissa, F., & Sakugawa, H. (2010). Negative effects of fluoranthene on the ecophysiology of tomato plants (Lycopersicon esculentum Mill): Fluoranthene mists negatively affected tomato plants. Chemosphere, 78(7), 877-884. Chemosphere 78(7): 877–884. https://doi.org/10.1016/j.chemosphere.2009.11.030

Pogorzelec, M., & Piekarska, K. (2018). Application of semipermeable membrane devices for long-term monitoring of polycyclic aromatic hydrocarbons at various stages of drinking water treatment. Science of The Total Environment, 631, 1431-1439. https://doi.org/10.1016/j.scitotenv.2018.03.105

Pretorius, T. R., Charest, C., Kimpe, L. E., & Blais, J. M. (2018). The accumulation of metals, PAHs and alkyl PAHs in the roots of Echinacea purpurea. PloS one, 13(12), e0208325. https://doi.org/10.1371/journal.pone.0208325

Ramel, F., Birtic, S., Cuiné, S., Triantaphylidès, C., Ravanat, J. L., & Havaux, M. (2012). Chemical quenching of singlet oxygen by carotenoids in plants. Plant physiology, 158(3), 1267-1278. https://doi.org/10.1104/pp.111.182394

Reynoso-Cuevas, L., Gallegos-Martínez, M. E., Cruz-Sosa, F., & Gutiérrez-Rojas, M. (2008). In vitro evaluation of germination and growth of five plant species on medium supplemented with hydrocarbons associated with contaminated soils. Bioresource technology, 99(14), 6379-6385. https://doi.org/10.1016/j.biortech.2007.11.074

Sabir, P., Ashraf, M., & Akram, N. A. (2011). Accession variation for salt tolerance in proso millet (Panicum miliaceum L.) using leaf proline content and activities of some key antioxidant enzymes. Journal of Agronomy and Crop Science, 197(5), 340-347. https://doi.org/10.1111/j.1439-037X.2011.00471.x

Salehi-Lisar, S. Y., & Deljoo, S. (2015). Physiological effect of phenanthrene on Triticum aestivum, He Ha nth us annus and Medicago sativa. EurAsian Journal of BioSciences, 9(1), 29-37. https://doi.org/10.5053/ejobios.2015.9.0.4

Singh-Tomar, R., & Jajoo, A. (2013). Alteration in PS II heterogeneity under the influence of polycyclic aromatic hydrocarbon (fluoranthene) in wheat leaves (Triticum aestivum). Plant Science, 209, 58-63. https://doi.org/10.1016/j.plantsci.2013.04.007

Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1

Smith, M. J., Lethbridge, G., & Burns, R. G. (1997). Bioavailability and biodegradation of polycyclic aromatic hydrocarbons in soils. FEMS Microbiology Letters, 152(1), 141-147. https://doi.org/10.1111/j.1574-6968.1997.tb10420.x

Somtrakoon, K., & Chouychai, W. (2013). Phytotoxicity of single and combined polycyclic aromatic hydrocarbons toward economic crops. Russian journal of plant physiology, 60(1), 139-148. https://doi.org/10.1134/S1021443712060155

Sverdrup, L. E., Krogh, P. H., Nielsen, T., Kjær, C., & Stenersen, J. (2003). Toxicity of eight polycyclic aromatic compounds to red clover (Trifolium pratense), ryegrass (Lolium perenne), and mustard (Sinapsis alba). Chemosphere, 53(8), 993-1003. https://doi.org/10.1016/S0045-6535(03)00584-8

Tian, L., Yin, S., Ma, Y., Kang, H., Zhang, X., Tan, H., ... & Liu, C. (2019). Impact factor assessment of the uptake and accumulation of polycyclic aromatic hydrocarbons by plant leaves: Morphological characteristics have the greatest impact. Science of the Total Environment, 652, 1149-1155. https://doi.org/10.1016/j.scitotenv.2018.10.357

Tomar, R. S., & Jajoo, A. (2014). Fluoranthene, a polycyclic aromatic hydrocarbon, inhibits light as well as dark reactions of photosynthesis in wheat (Triticum aestivum). Ecotoxicology and Environmental Safety, 109, 110-115. https://doi.org/10.1016/j.ecoenv.2014.08.009

Wei, H., Song, S., Tian, H., & Liu, T. (2014). Effects of phenanthrene on seed germination and some physiological activities of wheat seedling. Comptes Rendus Biologies, 337(2), 95-100. https://doi.org/10.1016/j.crvi.2013.11.005

Wiłkomirski, B., Jabbarov, Z. A., Abdrakhmanov, T. A., Vokhidova, M. B., Jabborov, B. T., Fakhrutdinova, M. F., ... & Abdullayeva, Y. D. (2018). Polycyclic Aromatic Hydrocarbons (PAHs) in Natural and Anthropogenically Modified Soils (A Review). Biogeosystem Technique, (5), 229-243. https://doi.org/10.13187/bgt.2018.2.229

Wilcke, W., Müller, S., Kanchanakool, N., Niamskul, C., & Zech, W. (1999). Polycyclic aromatic hydrocarbons in hydromorphic soils of the tropical metropolis Bangkok. Geoderma, 91(3-4), 297-309. https://doi.org/10.1016/S0016-7061(99)00012-9

Winterbourn, C. C., McGrath, B. M., & Carrell, R. W. (1976). Reactions involving superoxide and normal and unstable haemoglobins. Biochemical Journal, 155(3), 493-502. https://doi.org/10.1042/bj1550493




DOI: http://dx.doi.org/10.14720/aas.2021.117.2.1987

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