Primerjava regeneracije poganjkov in korenin pri miniaturni ločni vrtnici (Rosa hybrida L.) in damaščanski vrtnici (R. damascena Mill.) v mikrokulturi
Povzetek
Miniaturna lončna vrtnica in damaščanska vrtnica sta pomembni komercialni sorti med okrasnimi rastlinami. Za razmnoževanje vrtnic se najbolj pogosto uporabljajo poganjki iz korenin, a je njihova rast počasna in sezonsko odvisna. V raziskavi je bilo preučevano razmnoževanje teh dveh sort z izsečki nodijev v in vitro kulturah. Za regeneracijo poganjkov in korenin je bilo uporabljeno 16 in 24 različnih medijev. Zalistni brsti so odgnali prej pri miniaturni kot pri damaščanski vrtnici. Zasnova in rast poganjkov sta se pri miniaturni vrtnici pojavili 5 in 7 dni po sadnji in 16 ter 38 pri damaščanski vrtnici. Največje število poganjkov je bilo pri miniaturni vrtnici v medijih 3 in 7 in v mediju 16 pri damaščanski vrtnici. Ti trije mediji so bili prepoznani kot optimalni, sa je bila v njih dosežena 100 % tvorba poganjkov. V teh medijih sta bili zasnova in rast korenin pri miniaturni vrtnici zgodnejši (po 78 in 92 dneh) kot pri damaščanski vrtnici (po 125 in 138 dneh). Uspešno ukoreninjenje se je za miniaturno vrtnico pojavilo v treh medijih in le v dveh za damaščansko vrtnico. Pogostnost ukoreninjenja je bila večja v polovičnih tekočih MS medijih kot v drugih. Zaključimo lahko, da se miniaturna lončna vrtnica kot moderna vrsta razmnožuje lažje kot starinska damaščanska vrtnica.
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Abidin, N., and Metali, F. (2015). Effects of different types and concentrations of auxins on juvenile stem cuttings for propagation of potential medicinal Dillenia suffruticosa martelli shrub. Research Journal of Botany, 10(3), 73-87. https://doi.org/10.3923/rjb.2015.73.87
Ahmadian, E., Lolaei, A., Mobasheri, S., Bemana, R. (2013). Investigation of importance parameters of plant tissue (review). International Journal of Agriculture and Crop Sciences, 5(8), 900-905.
Alsemaan, T. (2013). Micro-propagation of Damask Rose (Rosa damascena Mill.) cv. Almarah. International Journal of Agricultural Research, 8(4), 172-177. https://doi.org/10.3923/ijar.2013.172.177
Asadi, A.A., Vedadi, C., Rahimi, M., Naserian, B. (2009). Effect of plant growth hormones on root and shoot regeneration in rose (Morrasia) under in-vitro conditions. Bioscience Research, 6(1), 40-45.
Badzian, T., Hennen, G.R., Fotyma-Kern, J. (1991). In vitro rooting of clonal propagated miniature rose cultivars. In International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement, 289, 329-330. https://doi.org/10.17660/ActaHortic.1991.289.81
Bidabadi, S.S., Jain, S. M. (2020). Cellular, molecular, and physiological aspects of in vitro plant regeneration. Plants, 9(6), 702. https://doi.org/10.3390/plants9060702
Brailko, V.A., Plugatar, S.A., Pilipchuk, T.I., Plugatar, Y.V., Mitrofanova, I.V. (2017). Morphological and physiological features of the miniature rose cultivar Risen Shine under long time culture in vitro and in vivo. In VII International Symposium on Production and Establishment of Micropropagated Plants, 1224, 139-144. https://doi.org/10.17660/ActaHortic.2018.1224.19
Cai, Z., Jing, X., Tian, X., Jiang, J., Liu, F., Wang, X. (2015). Direct and indirect in vitro plant regeneration and the effect of brassinolide on callus differentiation of Populus euphratica. South African Journal of Botany, 97, 143-148. https://doi.org/10.1016/j.sajb.2015.01.006
Davoudi Pahnekolayi, M., Samiei, L., Tehranifar, A., Shoor, M. (2015). The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.). Journal of Plant Molecular Breeding, 3(1), 61-71.
Edwin, F.G., Paul, D.Sh. (1984). Plant Propagation by Tissue Culture. Handbook and Directory of Comerical Laboratories. Exegetics Ltd., Eversley, Basingstoke, Hants, UK.
Ginova, A., Tsvetkov, I., kondakova, V. (2012). Rosa damascene Mill. An overview for evaluation of propagation methods. Bulgarian Journal of Agricultural Science, 18, 545-556.
Gubis, J., Lajchova, Z., Farago, J. Jurekova, Z. (2003). Effect of genotype and explant type on shoot regeneration in tomato (Lycopersicon esculentum) in vitro. Czech Journal of Genetics and Plant Breeding, 39(1), 9-14. https://doi.org/10.17221/3715-CJGPB
Hobbie, L., McGovern, M., Hurwitz, L.R., Pierro, A., Liu, N.Y., Bandyopadhyay, A., Estelle, M. (2000). The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development. Development, 127(1), 23-32. https://doi.org/10.1242/dev.127.1.23
Jabbarzadeh, Z., Khosh-Khui, M. (2005). Factors affecting tissue culture of Damask rose (Rosa damascena Mill.). Scientia Horticulturae, 105(4), 475-482. https://doi.org/10.1016/j.scienta.2005.02.014
Kadhimi, A.A., Alhasnawi, A.N., Mohamad, A., Yusoff, W.M.W., Zain, C. (2014). Tissue culture and some of the factors affecting them and the micropropagation of strawberry. Life Science Journal, 11(8), 484-493.
Khaleghi, A., Khadivi, A. (2020). Morphological characterization of Damask rose (Rosa× damascena) germplasm to select superior accessions. Genetic Resources and Crop Evolution, 67(8), 1981-1997. https://doi.org/10.1007/s10722-020-00954-z
Khaskheli, A.J., Khaskheli, M.I., Khaskheli, M.A., Shar, T., Ahmad, W., Lighari, U.A., Khaskheli, M.A., Khaskheli, A.A. and Makan, F.H. (2018). Proliferation, multiplication and improvement of micro-propagation system for mass clonal production of rose through shoot tip culture. American Journal of Plant Sciences, 9, 296-310. https://doi.org/10.4236/ajps.2018.92024
Kim C.K., Oh J.Y., Jee S.O., Chung J.D. (2003). In vitro micropropagation of Rosa hybrid L. Plant Biotechnology, 5, 115-119.
Kirichenko, E.B., Kuz-Mina, T.A., Kataeva, N.V. (1991). Factors in optimizing the multiplication of ornamental and essential oil roses in vitro. Byulleten-Glavnogo-Botanicheskogo Sada, 159, 61–67.
Ma, Y., Byrne, D. H., Chen, J. (1996). Propagation of rose species in vitro. In vitro Cellular and Developmental Biology - Plant, 32(2), 103-108. https://doi.org/10.1007/BF02823139
Mahmoudi Noodezh H.M., Moieni, A., Baghizadeh, A. (2012). In vitro propagation of the Damask rose (Rosa damascena Mill.), In Vitro Cellular and Developmental Biology – Plant, 48(5), 530-538. https://doi.org/10.1007/s11627-012-9454-z
Muiruri, S.N., Mweu, C.M. and Nyende, B.A. (2011). Micropropagation protocols using nodal explants of selected rose (Rosa hybrida) cultivars. African Journal of Horticultural Science, 4, 60-65.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nikbakht, A., Kafi, M., Mirmasoudi, M., Babalar, M. (2005). Micropropagation of damask rose (Rosa damascena Mill.) cvs Azaran and Ghamsar. International Journal of Agriculture and Biology, 7(4), 535-538.
Ozkan, G., Sagdic, O., Baydar, N.G., Baydar, H. (2004). Note: Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Science and Technology International, 10(4), 277-281. https://doi.org/10.1177/1082013204045882
Pati, P.K., Rath, S.P., Sharma, M., Sood, A., Ahuja, P.S. (2006). In vitro propagation of rose- a review. Biotechnology advances, 24(1), 94-114. https://doi.org/10.1016/j.biotechadv.2005.07.001
Pati, P.K., Kaur, N., Sharma, M. and Ahuja, P.S. (2010). In vitro propagation of rose. In Protocols for in vitro Propagation of Ornamental Plants, 163-176. https://doi.org/10.1007/978-1-60327-114-1_16
Pierik, R.L.M. (1991). Horticulture new technologies and applications proceeding of the international seminar on new frontiers in horticulture. Current Plant Science and Biotechnology in Agriculture, 12, 141–153. https://doi.org/10.1007/978-94-011-3176-6_23
Tarrahi, R., Rezanejad, F. (2013). Callogenesis and production of anthocyanin and chlorophyll in callus cultures of vegetative and floral explants in Rosa gallica and R. hybrida (Rosaceae). Turkish Journal of Botany, 37(6), 1145-1154. https://doi.org/10.3906/bot-1205-42
Thi, K.O., Khai, A.A., Lwin, K.M. (2008). Micropropagation of Rose (Rosa hubrida spp.) by in vitro culture technique. GMSARN International Conference on Sustainable Development: Issues and Prospects forthe GMS, 12-14.
Tibkwang, A., Junkasiraporn, S., Chotikadachanarong, K. (2018). Effects of Cytokinnin and Sucrose on Tissue Culture of Rosa chinensis. Burapha Science Journal, 23(2), 712-721.
Younis, A., Riaz, A., Javaid, F., Ahsan, M., Tariq, U., Aslam, S. Majeed, N. (2015). Influence of various growing substrates on growth and flowering of potted miniature rose cultivar “Baby Boomer”. Specialty Journal of Agricultural Sciences, 1(2), 28-33.
DOI: http://dx.doi.org/10.14720/aas.2023.119.1.2380
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