Pospeševanje tvorbe poganjkov in ovrednotenje elicitacijskih učinkov na anatomske spremembe navideznih stebel in proti maščobne peroksidacijske aktivnosti kurkume (Curcuma mangga Val.)

Fariz ABRAHAM, Lai-Keng CHAN, Gunawan INDRAYANTO, Peng Lim BOEY

Povzetek


Kurkuma (Curcuma mangga Val.) vsebuje številne bioaktivne snovi, ki se uporabljajo pri tradicionalnem obravnavanju številnih zdravstvenih težav in obolenj. Počasen način njenega razmnoževanja povzroča njeno pomanjkanje glede na veliko povpraševanje na trgu. Do polovice vzdolžno zarezani stebelni izsečki so stoodstotno povečali število poganjkov v primerjavi z nezarezanimi s tvorbo povprečno 6,6 poganjkov na izseček, če so bili gojeni navpično ali vodoravno v MS gojišču, obogatenim z 2,0 mg l-1 BA in 0,5 mg l-1 NAA. Biotično vzpodbujanje s 3,5 mg l-1 ali 5,0 mg l-1 izvlečka kvasa v kombinaciji z 150 mg l-1 hitozana in 3,5 mg l-1 izvlečka kvasa ni pospešilo tvorbe poganjkov ampak je pokazalo malo manjšo antiperoksidacijsko aktivnost v primerjavi s kvercetinom, močnim rastlinskim flavonoidnim antioksidatom in butil hidroksi toluenom (BHT), komercialnim zaščitnim sredstvom, ki sta bila uporabljena kot pozitivna kontrola. Uporaba absolutnega etanola kot negativne kontrole ni pokazala nobene antiperoksidacijske aktivnosti. Biotično vzpodbujanje nastanka rastlinic korkume s podobnimi eliciatorji je povzročilo anatomske spremembe v nastajajočih navideznih steblih z zmanjšanjem števila tankih lignificiranih celic ksilema in prisotnostjo kritalnih kopuč, domnevno iz kalcijevega oksalata, v celicah primarne skorje, ki so imele zelo tanko celično steno.


Ključne besede


antiperoksidacijska aktivnost za maščobe; hitozan; kurkuma; navidezna stebla; tvorba poganjkov; izvlečki kvasa

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2022.118.2.2416

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Avtorske pravice (c) 2022 Fariz ABRAHAM, Lai Keng CHAN, Gunawan INDRAYANTO, Peng Lim BOEY

 

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