VELIKA KONCENTRACIJA SALICILNE KISLINE SPREMINJA PRI JEČMENU FOTOSINTEZNI, S FOTOSISTEMOM II POVEZAN ELEKTRONSKI PRETOK

Ghader Habibi, Atousa Vaziri

Povzetek


V raziskavi so bili preučevani učinki dodajanja salicilne kisline (0.5 in 5 mM) na aktivnost fotosistema II (PSII) pri 4-tedne starih kalicah ječmena (Hordeum vulgare‘Bahman’) z meritvami fluorescence (OJIP) klorofila a (Chla). Nobenih sprememb v vsebnosti klorofila in karotenoidov kot tudi ne sprememb v fluorescenci ni bilo opaznih po 24 urah dodajanja obeh koncentracij salicilne kisline. Po petih dneh so se v kalicah ječmena pri dodani manjši koncentraciji salicilne kisline (0.5 mM) povečali aktivnost PSII, vsebnost Chl b in karotenoidov. Nasprotno sta se pet dni po obravnavanju s 5 mM salicilno kislino značilno zmanjšala učinkovitost PSII (Fv/Fm) in PIABS indeks kot indikatorja zgradbe in delovanja PSII. Zmanjšanje Fv/Fm in PIABS je bilo povezano z zmanjšanjem vsebnosti klorofila b in karotenoidov ter z manjšimi vrednostimi komponent fotosintezne elektronske verige, vključno s elektronskim pretokom (φEo) in z njim povezano aktivnostjo kompleksa, ki sprošča kisik (Fv/Fo). Pri spremljanju povečanja fluorescence klorofila a od začetne “O” na največjo vrednost “P” je bilo opazno njeno dramatično povečanje v fazi “OJ”, kar je soupadalo s povečano fotoredukcijo QA kot posledica blokade fotosintezenega elektronskega pretoka. Raziskava dokazuje, da večja koncentracija salicilne kisline povzroči poškodbe na večih mestih PSII.

Ključne besede


fotosintezna barvila; fotosintezni elektronski pretok; Hordeum vulgare ‘Bahman’, OJIP prehodna fluorescenca; salicilna kislina

Celotno besedilo:

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Literatura


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DOI: http://dx.doi.org/10.14720/aas.2017.109.2.22

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