Euphorbia bupleuroides Desf. latex as biopesticide against the red flour beetle (Tribolium castaneum [Herbst, 1797]) and khapra beetle (Trogoderma granarium Everts, 1898)

Imene BRIK, Naama FRAH


Laboratory evaluation of Euphorbia bupleuroides latex as biopesticide against the red flour beetle (Tribolium castaneum) and khapra beetle (Trogoderma granarium) were evaluated at ambient temperature. The insecticidal activity of latex was determined by direct contact application. Different concentrations were prepared by dilution of 2.5, 5.0, 7.0 and 10.0 µl of latex into 0.1 ml acetone. 1 µl was pumped regularly in the thorax of different insects. The latex of E. bupleuroides showed insecticidal activity against T. granarium and T. castaneum. High levels of mortality were associated with the increase in the concentration and time of exposure as well. T. granarium adults are generally more prone to latex insecticidal effects than T. castaneum adults. After 6 days of exposure to E. bupleuroides latex, the LC50 recorded was 14.12 µl for T. granarium adults, and 14.7 µl for T. castaneum. LC90 numbers, on the other hand, were 38.8 µl for the former, and 51.44 µl for the latter.


Euphorbia bupleuroides; biopesticide; latex; Tribolium castaneum; Trogoderma granarium; mortality

Full Text:



Abbott, W.S. (1925). A method of computing effectiveness of an insecticide. Journal of Economic Entomology, 18, 265–267.

Aichour, S., Haba, H., Benkhaled, M., Harakat, D., Lavaud, C. (2014). Terpenoids and other constituents from Euphorbia bupleuroides. Photochemistry Letters, 10, 198-203.

AL-Younis, N. K. and Abdullah, A. F. (2009). Isolation and antibacterial evaluation of plant extracts from some medicinal plants in Kurdistan region, J. Duhok University,12(1), 250-255.

Arthur, F.H. (1996). Grain protectants: Current status and prospects for the future. Journal of Stored Products Research, 32, 293–302.

Aslanturk, O.S., C¸elik, T.A.(2013). Antioxidant, cytotoxic and apoptotic activities of extracts from medicinal plant Euphorbia platyphyllos L. Journal of Medicinal Plants Research, 7, 1293–1304.

Biesboer, D.D. and Mahlberg, P.G., (1979). The effect of medium modification and selected precursors on sterol production by short-term callus cultures of Euphorbia tirucalli. Journal of Natural Products, 42 (6), 648-657.

Cateni, F., Zilic, J., Zacchigna, M., Procida, G.( 2010). Cerebrosides with antiprolifera-tive activity from Euphorbia peplis L. Fitoterapia 81, 97–103.

Chang, K.S., and Anh, Y.J. (2002). Fumigant activity of (E)-anethole identified in Illicium verum against Blatella germanica. Pest Management Science, 58, 161–166.

De Silva, W.A.P.P., Manuweera, G.K., Karunaratne, S.H.P.P.(2008). Insecticidal activity of Euphorbia antiquorum L. latex and its preliminary chemical analysis. Journal of the National Science Foundation of Sri Lanka, 36, 15-23.

Finney, D.J.(1971). Probit Analysis, Cambridge University Press, Cambridge, England, ISBN: 052108041X Wheeler, M.W.

Gayar, F.H., Shazli, A.Y. and Abbassy, M.A. (1971). Toxicity of Euphorbia peplus L.(Euphorbaceae) to insects. Journal of Applied Entomology, 68, issue 1-4, pp. 63-65.

Geng, Z. F., Liu, Z. L., Wang, C. F., Liu, Q. Z., Shem, S.M., Lin, Z.M., Du, S.S., Deng, Z.W.(2011). Feeding deterrents against two grain storage insects from Euphorbia fischeriana. Molecules, 16, 466-476.

Giner, J.L., Berkowitz, J.D., Andersson, T.( 2000). Nonpolar components of the latex of Euphorbia peplus. Journal of Natural Products, 63, 267–269.

Jain, R., Jindal, C., Sing, S., Datta, A. (2008). Phormaceutical compositions comprising an extract of E. prostrata US patent/ patent no. US 7, 731,412 B2 May 13.

Khan, A.Q., Kazmi, S.N.H., Ahmed, Z., Malik, A.(1989). Euphorcinol -a new pentacyclic triterpene from Euphorbia tirucalli. Planta Medica, 55, 290-291.

Kurpadanam, V.G, Srim, G. (1999). Cyclonivulialol a new cycloartane epoxy triterpenoids from Euphorbia buch-ham. Indian journal of chemistry, 30,989-993.

Lee, C.H., Jeon, J., Lee, S. and Lee, H., (2010). Insecticidal properties of Euphorbiaceae: Sebastiania corniculata derived 8-hydroxyquinoline and its derivatives against three plant hopper species (Hemiptera: Delphacidae). Journal of the Korean Society for Applied Biological Chemistry, 53, 464-469.

Lima, M.G.A., Maia, I.C.C., Sousa, B.D., Morais, S.M., Freitas, S.M. (2006) . Effect of stalk and leaf extracts from Euphorbiaceae species on Aedes aegypti (Diptera : Culicidae) larvae.Revista do Instituto de Medicina Tropical de São Paulo, 48(4),211-214.

Mwine, J., Damme, P. and Jumba, F., (2010). Evaluation of larvicidal properties of the latex of Euphorbia tirucalli L. (Euphorbiaceae) against larvae of Anopheles mosquitoes. Journal of Medicinal Plants Research, 4(19), 1954-1959.

Quezel, P., and Santa, S. (1963). Nouvelle Flore de l’Algérie et des régions désertiques méridionales, vol. 1–2. CNRS, Paris

Rafael, E. (2001). Botanical insecticides. www.ipmworld. Accessed on 12 October 2001.

Robertson, J.L., Savin, N.E., Russell, R.M. and Preisler, H.K.(2007). Bioassays with arthropods. CRC press. ISBN: 9780849323317

Saito, T., and Hama, H .(2000). Carboxylesterase isozymes responsible for organophosphate resistance in the cotton aphid, Aphis gossypy Glover (Homoptera: Aphididae). Applied Entomology and Zoology, 35, 171-175.


Singh, P., and Singh, A.( 2012). Evaluation of latex extract of Euphorbia royleana for its piscicidal and muricidal activities. World Journal of Agricultural Sciences, 8, 520-524.

Tanaka, R., Kasubuchi, K., Kita, S., Matsunaga, S.(1999). Obtusifoliol and related steroids from the whole herb of Euphorbia chamaesyce. Phytochemistry, 51, 457–463.

Vanderplank, F (1945). Insecticidal Properties of Euphorbia Extracts. Nature, 156, 782.

Vasas, A., Sulyok, E., Martins, A., Redei, D., Forgo, P., Kele, Z., Zupko, I., Molnar, J., Pinke, G., Hohmann, J. (2012). Cyclomyrsinane and premyrsinane diterpenes from Euphorbia falcata modulate resistance of cancer cells to doxorubicin. Tetrahedron, 68, 1280–1285. doi:10.1016/j.tet.2011.11.039

Vimal, J.B., and Das, S.S.M.(2014). Euphorbia antiquorum latex and its mosquitocidal potency against Aedes aegypti.Journal of Entomology and Zoology Studies, 2 (6), 267-269.

Webster, G.L., 1994. Classification of the Euphorbiaceae. Annals of the Missouri Botanical Garden, Vol. 81, No. 1 pp. 3-32.

Wink, M.(1993). Production and application of phytochemicals from an agricultural perspective. In: van Beek TA, Breteler H, editors. Phytochemistry and Agriculture. Oxford, UK: Clarendon Press 34: pp. 171–213.

Yamamoto, Y., Mizuguchi, R. and Yamada, Y., (1981). Chemical constituents of cultured cells of Euphorbia tirucalli and E. milii. Plant Cell Reports, 1: 29



  • There are currently no refbacks.

Copyright (c) 2021 Imene Brik

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Acta agriculturae Slovenica is an Open Access journal published under the terms of the Creative Commons CC BY License.


eISSN 1854-1941